Gene transfer directly demonstrates a role for TCR Vα elements in superantigen recognition

David Donson, Heriberto Borrero, Matthew Rutman, Robert Pergolizzi, Norika O Malhado-Chang, Stuart Macphail

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Recent structure-function studies of ours and others indicating that regions of the TCR other than Vβ are involved in the TCR-superantigen (SAg)-MHC class II trimolecular interaction were correlative; thus, while the conclusions were persuasive, they were not unequivocal. The transfecfion experiments described in this report show that 1) responsiveness to staphylococcal enterotoxin B in Vβ6 T cells was transferred by a Vα4- but not by Vα8- and Vα10-containing α-chain cDNA constructs, 2) responsiveness was not transferred by a chimeric α-chain construct containing the N and J regions from a responsive T hybrid clone and the Vα10 Vα region from a nonresponsive clone, and 3) responsiveness was transferred by a chimeric α-chain construct in which most of the Vα region (from the N terminus to the C-terminal end of the complementarity-determining region 2) was derived from the Vα4 α-chain of a responsive T hybrid and the rest (framework 3, N, and J) from the Vα8 α-chain of a nonresponsive T hybrid. Thus, these data provide the first direct evidence for a specific SAg response facilitating activity in a defined Vα segment and map this activity N-terminal of framework region 3. Furthermore, the diversity in the α- and β-chain functional regions of a panel of staphylococcal enterotoxin B-responsive Vβ6 T hybrid clones excludes a stringent corequirement for a oarticular iunctional region for the Vα4 segment to mediate its facilitating activity. Finally, a model postulating a universal role for Vα elements in TCR recognition of SAg is presented.

Original languageEnglish (US)
Pages (from-to)5229-5236
Number of pages8
JournalJournal of Immunology
Volume158
Issue number11
StatePublished - 1997
Externally publishedYes

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Superantigens
Clone Cells
Genes
Complementarity Determining Regions
Complementary DNA
T-Lymphocytes
staphylococcal enterotoxin B

ASJC Scopus subject areas

  • Immunology

Cite this

Donson, D., Borrero, H., Rutman, M., Pergolizzi, R., Malhado-Chang, N. O., & Macphail, S. (1997). Gene transfer directly demonstrates a role for TCR Vα elements in superantigen recognition. Journal of Immunology, 158(11), 5229-5236.

Gene transfer directly demonstrates a role for TCR Vα elements in superantigen recognition. / Donson, David; Borrero, Heriberto; Rutman, Matthew; Pergolizzi, Robert; Malhado-Chang, Norika O; Macphail, Stuart.

In: Journal of Immunology, Vol. 158, No. 11, 1997, p. 5229-5236.

Research output: Contribution to journalArticle

Donson, D, Borrero, H, Rutman, M, Pergolizzi, R, Malhado-Chang, NO & Macphail, S 1997, 'Gene transfer directly demonstrates a role for TCR Vα elements in superantigen recognition', Journal of Immunology, vol. 158, no. 11, pp. 5229-5236.
Donson, David ; Borrero, Heriberto ; Rutman, Matthew ; Pergolizzi, Robert ; Malhado-Chang, Norika O ; Macphail, Stuart. / Gene transfer directly demonstrates a role for TCR Vα elements in superantigen recognition. In: Journal of Immunology. 1997 ; Vol. 158, No. 11. pp. 5229-5236.
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