Purpose. In multiple orders of animals, the ocular lens assembles a cytoskeletal structure termed the Beaded Filament (BF). DNA sequence analysis reveals similary to, yet significant departures from, Intermediate Filament (IF) proteins. We have determined the human CP 115 cDNA sequence and investigated CP 115 gene structure to determine evolutionary and developmental relationships between CP 115 and IFs. Methods. PCR amplification of cDNA and genomic DNA has been utilized to clone human CP 115 sequences. Additionally, a human BAC clone containing CP 115 sequences was isolated for the subcloning of CP 115 genomic fragments. Results. Our DNA sequencing data reveal conservation of the primary structure of CP 115 as compared to bovine, chicken and rat. CP 115 gene structure reveals conservation of some IF introns but not complete conservation of the intron structure of any one class of IF protein. Conclusion. In contrast to CP 49, the other identified BF protein, the gene structure of CP 115 does not conform to a single existing class of IF proteins. This data, combined with the predicted amino acid sequence of the protein, leads to the conclusion that CP 115 and possibly CP 49 define a new class of IF proteins developmentally regulated to the lens fiber cell.
|Original language||English (US)|
|Journal||Investigative Ophthalmology and Visual Science|
|State||Published - 1997|
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