Gene regulation in parthenocarpic tomato fruit

Federico Martinelli, Sandra L. Uratsu, Russell L. Reagan, Ying Chen, David Tricoli, Oliver Fiehn, David M Rocke, Charles S. Gasser, Abhaya M. Dandekar

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Parthenocarpy is potentially a desirable trait for many commercially grown fruits if undesirable changes to structure, flavour, or nutrition can be avoided. Parthenocarpic transgenic tomato plants (cv MicroTom) were obtained by the regulation of genes for auxin synthesis (iaaM) or responsiveness (rolB) driven by DefH9 or the INNER NO OUTER (INO) promoter from Arabidopsis thaliana. Fruits at a breaker stage were analysed at a transcriptomic and metabolomic level using microarrays, real-time reverse transcription-polymerase chain reaction (RT-PCR) and a Pegasus III TOF (time of flight) mass spectrometer. Although differences were observed in the shape of fully ripe fruits, no clear correlation could be made between the number of seeds, transgene, and fruit size. Expression of auxin synthesis or responsiveness genes by both of these promoters produced seedless parthenocarpic fruits. Eighty-three percent of the genes measured showed no significant differences in expression due to parthenocarpy. The remaining 17% with significant variation (P<0.05) (1748 genes) were studied by assigning a predicted function (when known) based on BLAST to the TAIR database. Among them several genes belong to cell wall, hormone metabolism and response (auxin in particular), and metabolism of sugars and lipids. Up-regulation of lipid transfer proteins and differential expression of several indole-3-acetic acid (IAA)- and ethylene-associated genes were observed in transgenic parthenocarpic fruits. Despite differences in several fatty acids, amino acids, and other metabolites, the fundamental metabolic profile remains unchanged. This work showed that parthenocarpy with ovule-specific alteration of auxin synthesis or response driven by the INO promoter could be effectively applied where such changes are commercially desirable.

Original languageEnglish (US)
Pages (from-to)3873-3890
Number of pages18
JournalJournal of Experimental Botany
Volume60
Issue number13
DOIs
StatePublished - Sep 2009

Fingerprint

Lycopersicon esculentum
Fruit
Indoleacetic Acids
tomatoes
parthenocarpy
fruits
auxins
Genes
genes
promoter regions
synthesis
genetically modified organisms
Ovule
hormone metabolism
Metabolomics
Metabolome
Genetically Modified Plants
metabolomics
spectrometers
transcriptomics

Keywords

  • Fruit quality
  • Fruit ripening
  • INO
  • Parthenocarpic
  • Seedless fruit
  • Tomato

ASJC Scopus subject areas

  • Plant Science
  • Physiology

Cite this

Martinelli, F., Uratsu, S. L., Reagan, R. L., Chen, Y., Tricoli, D., Fiehn, O., ... Dandekar, A. M. (2009). Gene regulation in parthenocarpic tomato fruit. Journal of Experimental Botany, 60(13), 3873-3890. https://doi.org/10.1093/jxb/erp227

Gene regulation in parthenocarpic tomato fruit. / Martinelli, Federico; Uratsu, Sandra L.; Reagan, Russell L.; Chen, Ying; Tricoli, David; Fiehn, Oliver; Rocke, David M; Gasser, Charles S.; Dandekar, Abhaya M.

In: Journal of Experimental Botany, Vol. 60, No. 13, 09.2009, p. 3873-3890.

Research output: Contribution to journalArticle

Martinelli, F, Uratsu, SL, Reagan, RL, Chen, Y, Tricoli, D, Fiehn, O, Rocke, DM, Gasser, CS & Dandekar, AM 2009, 'Gene regulation in parthenocarpic tomato fruit', Journal of Experimental Botany, vol. 60, no. 13, pp. 3873-3890. https://doi.org/10.1093/jxb/erp227
Martinelli F, Uratsu SL, Reagan RL, Chen Y, Tricoli D, Fiehn O et al. Gene regulation in parthenocarpic tomato fruit. Journal of Experimental Botany. 2009 Sep;60(13):3873-3890. https://doi.org/10.1093/jxb/erp227
Martinelli, Federico ; Uratsu, Sandra L. ; Reagan, Russell L. ; Chen, Ying ; Tricoli, David ; Fiehn, Oliver ; Rocke, David M ; Gasser, Charles S. ; Dandekar, Abhaya M. / Gene regulation in parthenocarpic tomato fruit. In: Journal of Experimental Botany. 2009 ; Vol. 60, No. 13. pp. 3873-3890.
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