Gene-protein correlation in single cells

Jaclyn J. Renfrow, Adrienne C. Scheck, Neil S. Dhawan, Paul J. Lukac, Hannes Vogel, James P. Chandler, Jeffrey J. Raizer, Griffith R. Harsh, Arnab Chakravarti, Markus Bredel

Research output: Contribution to journalArticlepeer-review

Abstract

We present a novel methodology combining traditional fluorescent in situ hybridization with an in situ protein detection technology called proximity ligation assay. This method has potential to perform a detailed analysis of the relationship between gene status and corresponding protein expression in cells and tissues. We demonstrate that the fluorescent in situ gene protein assay methodology is capable of resolving gene and protein patterns simultaneously on a cell-by-cell basis.

Original languageEnglish (US)
Pages (from-to)880-885
Number of pages6
JournalNeuro-Oncology
Volume13
Issue number8
DOIs
StatePublished - Aug 2011
Externally publishedYes

Keywords

  • Epidermal growth factor receptor
  • Fluorescence in situ gene protein assay
  • Fluorescence in situ hybridization
  • Gene amplification
  • Glioblastoma
  • Proximity ligation assay

ASJC Scopus subject areas

  • Oncology
  • Clinical Neurology
  • Cancer Research

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