Gene mdpC plays a regulatory role in the methyl-tert-butyl ether degradation pathway of Methylibium petroleiphilum strain PM1

Geetika Joshi, Radomir Schmidt, Kate M. Scow, Michael S. Denison, Krassimira R. Hristova

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Among the few bacteria known to utilize methyl tert-butyl ether (MTBE) as a sole carbon source, Methylibium petroleiphilum PM1 is a well-characterized organism with a sequenced genome; however, knowledge of the genetic regulation of its MTBE degradation pathway is limited. We investigated the role of a putative transcriptional activator gene, mdpC, in the induction of MTBE-degradation genes mdpA (encoding MTBE monooxygenase) and mdpJ (encoding tert-butyl alcohol hydroxylase) of strain PM1 in a gene-knockout mutant mdpC-. We also utilized quantitative reverse transcriptase PCR assays targeting genes mdpA, mdpJ and mdpC to determine the effects of the mutation on transcription of these genes. Our results indicate that gene mdpC is involved in the induction of both mdpA and mdpJ in response to MTBE and tert-butyl alcohol (TBA) exposure in PM1. An additional independent mechanism may be involved in the induction of mdpJ in the presence of TBA.

Original languageEnglish (US)
Article numberfnv029
JournalFEMS Microbiology Letters
Volume362
Issue number7
DOIs
StatePublished - 2015

Fingerprint

tert-Butyl Alcohol
Genes
Mixed Function Oxygenases
Gene Knockout Techniques
Gene Targeting
Reverse Transcriptase Polymerase Chain Reaction
Carbon
methyl tert-butyl ether
Genome
Bacteria
Mutation

Keywords

  • Degradation pathway
  • MdpC
  • mdpC- mutant
  • Methylibium petroleiphilum strain PM1
  • MTBE
  • Regulation

ASJC Scopus subject areas

  • Microbiology
  • Genetics
  • Molecular Biology

Cite this

Gene mdpC plays a regulatory role in the methyl-tert-butyl ether degradation pathway of Methylibium petroleiphilum strain PM1. / Joshi, Geetika; Schmidt, Radomir; Scow, Kate M.; Denison, Michael S.; Hristova, Krassimira R.

In: FEMS Microbiology Letters, Vol. 362, No. 7, fnv029, 2015.

Research output: Contribution to journalArticle

Joshi, Geetika ; Schmidt, Radomir ; Scow, Kate M. ; Denison, Michael S. ; Hristova, Krassimira R. / Gene mdpC plays a regulatory role in the methyl-tert-butyl ether degradation pathway of Methylibium petroleiphilum strain PM1. In: FEMS Microbiology Letters. 2015 ; Vol. 362, No. 7.
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