Gene families encoding transcription factors expressed in early development of Strongylocentrotus purpuratus

Meredith Howard-Ashby, Stefan C. Materna, Charles Brown, Lili Chen, R. Andrew Cameron, Eric H. Davidson

Research output: Contribution to journalArticle

111 Citations (Scopus)

Abstract

All genes encoding transcription factors of the bHLH, Nuclear Receptor, Basic Leucine Zipper, T-box, Smad, Sox, and other smaller families were identified in the Strongylocentrotus purpuratus genome by means of a permissive blast search of the genome using a database of known transcription factors. Phylogenetic trees were constructed for the major families, permitting a comparison of the regulatory protein repertoire of the sea urchin and other species. QPCR and whole mount in situ hybridization experiments revealed the temporal and spatial expression patterns of these genes during early development. These regulatory genes are initially expressed at a broad range of time points, and the large majority of genes of all families are expressed within the first 48 h of development. The observations suggest assignment of many regulatory genes to specific developmental sub-networks, including endomesodermal, oral, aboral, and apical.

Original languageEnglish (US)
Pages (from-to)90-107
Number of pages18
JournalDevelopmental Biology
Volume300
Issue number1
DOIs
StatePublished - Dec 1 2006
Externally publishedYes

Fingerprint

Strongylocentrotus purpuratus
Regulator Genes
Transcription Factors
Genome
Basic Helix-Loop-Helix Transcription Factors
Leucine Zippers
Sea Urchins
Cytoplasmic and Nuclear Receptors
Genes
In Situ Hybridization
Databases
Gene Expression
Proteins

Keywords

  • Basic zipper
  • bHLH
  • Development
  • Nuclear receptor
  • Sea urchin
  • smad
  • Sox
  • Transcription factor

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

Cite this

Gene families encoding transcription factors expressed in early development of Strongylocentrotus purpuratus. / Howard-Ashby, Meredith; Materna, Stefan C.; Brown, Charles; Chen, Lili; Cameron, R. Andrew; Davidson, Eric H.

In: Developmental Biology, Vol. 300, No. 1, 01.12.2006, p. 90-107.

Research output: Contribution to journalArticle

Howard-Ashby, Meredith ; Materna, Stefan C. ; Brown, Charles ; Chen, Lili ; Cameron, R. Andrew ; Davidson, Eric H. / Gene families encoding transcription factors expressed in early development of Strongylocentrotus purpuratus. In: Developmental Biology. 2006 ; Vol. 300, No. 1. pp. 90-107.
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