Gene expression profiling of mouse articular and growth plate cartilage

Shintaro Yamane, Ewana Cheng, Zongbing You, A Hari Reddi

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Articular cartilage is recalcitrant to repair and regeneration. Tissue engineering and regenerative medicine are potential strategies to treat the damage to articular cartilage. A thorough understanding of the gene expression profiles in articular cartilage and growth plate chondrocytes will be an important prerequisite for tissue engineering of cartilage. Regeneration is a recapitulation of embryonic development and morphogenesis. We used laser capture microdissection to capture the surface articular chondrocytes and the resting zone chondrocytes of growth plate from 14-day-old C57BL/6J mice. Total RNA was individually purified, pooled, and amplified by two rounds of in vitro transcription. Labeled cRNA probes were analyzed using the Affymetrix GeneChip® Mouse Genome 430 2.0 Array. We identified 107 genes that were highly expressed by the surface articular chondrocytes and 130 genes that were highly expressed by the resting zone chondrocytes of growth plate (≥ fivefold). The expression of major matrix proteins aggrecan and collagen II were similar, while several morphogens and growth factors were differentially expressed by the surface articular chondrocytes and the resting zone chondrocytes of growth plate. The results of this investigation will be of use in the evaluation of tissue engineered cartilage.

Original languageEnglish (US)
Pages (from-to)2163-2173
Number of pages11
JournalTissue Engineering
Volume13
Issue number9
DOIs
StatePublished - Sep 2007

Fingerprint

Growth Plate
Cartilage
Gene Expression Profiling
Chondrocytes
Gene expression
Joints
Articular Cartilage
Genes
Tissue engineering
Microdissection
Tissue Engineering
Regeneration
Bioelectric potentials
Transcription
RNA
Collagen
Laser Capture Microdissection
Aggrecans
Complementary RNA
Regenerative Medicine

ASJC Scopus subject areas

  • Biophysics
  • Cell Biology
  • Biotechnology

Cite this

Gene expression profiling of mouse articular and growth plate cartilage. / Yamane, Shintaro; Cheng, Ewana; You, Zongbing; Reddi, A Hari.

In: Tissue Engineering, Vol. 13, No. 9, 09.2007, p. 2163-2173.

Research output: Contribution to journalArticle

Yamane, Shintaro ; Cheng, Ewana ; You, Zongbing ; Reddi, A Hari. / Gene expression profiling of mouse articular and growth plate cartilage. In: Tissue Engineering. 2007 ; Vol. 13, No. 9. pp. 2163-2173.
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