Gene expression in blood changes rapidly in neutrophils and monocytes after ischemic stroke in humans: A microarray study

Yang Tang, Huichun Xu, Xinli Du, Lisa Lit, Wynn Walker, Aigang Lu, Ruiqiong Ran, Jeffrey Gregg, Melinda Reilly, Art Pancioli, Jane C. Khoury, Laura R. Sauerbeck, Janice A. Carrozzella, Judith Spilker, Joseph Clark, Kenneth R. Wagner, Edward C. Jauch, Dongwoo J. Chang, Piero Verro, Joseph P. BroderickFrank R Sharp

Research output: Contribution to journalArticle

215 Citations (Scopus)

Abstract

Ischemic brain and peripheral white blood cells release cytokines, chemokines and other molecules that activate the peripheral white blood cells after stroke. To assess gene expression in these peripheral white blood cells, whole blood was examined using oligonucleotide microarrays in 15 patients at 2.4±0.5, 5 and 24 h after onset of ischemic stroke and compared with control blood samples. The 2.4-h blood samples were drawn before patients were treated either with tissue-type plasminogen activator (tPA) alone or with tPA plus Eptifibatide (the Combination approach to Lysis utilizing Eptifibatide And Recombinant tPA trial). Most genes induced in whole blood at 2 to 3 h were also induced at 5 and 24 h. Separate studies showed that the genes induced at 2 to 24 h after stroke were expressed mainly by polymorphonuclear leukocytes and to a lesser degree by monocytes. These genes included: matrix metalloproteinase 9; S100 calcium-binding proteins P, A12 and A9; coagulation factor V; arginase I; carbonic anhydrase IV; lymphocyte antigen 96 (cluster of differentiation (CD)96); monocarboxylic acid transporter (6); ets-2 (erythroblastosis virus E26 oncogene homolog 2); homeobox gene Hox 1.11; cytoskeleton-associated protein 4; N-formylpeptide receptor; ribonuclease-2; N-acetylneuraminate pyruvate lyase; BCL6; glycogen phosphorylase. The fold change of these genes varied from 1.6 to 6.8 and these 18 genes correctly classified 10/15 patients at 2.4 h, 13/15 patients at 5 h and 15/15 patients at 24 h after stroke. These data provide insights into the inflammatory responses after stroke in humans, and should be helpful in diagnosis, understanding etiology and pathogenesis, and guiding acute treatment and development of new treatments for stroke.

Original languageEnglish (US)
Pages (from-to)1089-1102
Number of pages14
JournalJournal of Cerebral Blood Flow and Metabolism
Volume26
Issue number8
DOIs
StatePublished - Aug 14 2006

Fingerprint

Monocytes
Neutrophils
Stroke
Gene Expression
Leukocytes
Genes
Plasminogen Activators
N-acetylneuraminate lyase
Lymphocyte Antigen 96
Carbonic Anhydrase IV
Monocarboxylic Acid Transporters
Glycogen Phosphorylase
Arginase
Calcium-Binding Proteins
compound A 12
Factor V
Homeobox Genes
Matrix Metalloproteinase 9
Tissue Plasminogen Activator
Ribonucleases

Keywords

  • Genomics
  • Inflammation
  • Ischemia
  • Leukocytes
  • Microarrays
  • Neutrophils

ASJC Scopus subject areas

  • Endocrinology
  • Neuroscience(all)
  • Endocrinology, Diabetes and Metabolism

Cite this

Gene expression in blood changes rapidly in neutrophils and monocytes after ischemic stroke in humans : A microarray study. / Tang, Yang; Xu, Huichun; Du, Xinli; Lit, Lisa; Walker, Wynn; Lu, Aigang; Ran, Ruiqiong; Gregg, Jeffrey; Reilly, Melinda; Pancioli, Art; Khoury, Jane C.; Sauerbeck, Laura R.; Carrozzella, Janice A.; Spilker, Judith; Clark, Joseph; Wagner, Kenneth R.; Jauch, Edward C.; Chang, Dongwoo J.; Verro, Piero; Broderick, Joseph P.; Sharp, Frank R.

In: Journal of Cerebral Blood Flow and Metabolism, Vol. 26, No. 8, 14.08.2006, p. 1089-1102.

Research output: Contribution to journalArticle

Tang, Y, Xu, H, Du, X, Lit, L, Walker, W, Lu, A, Ran, R, Gregg, J, Reilly, M, Pancioli, A, Khoury, JC, Sauerbeck, LR, Carrozzella, JA, Spilker, J, Clark, J, Wagner, KR, Jauch, EC, Chang, DJ, Verro, P, Broderick, JP & Sharp, FR 2006, 'Gene expression in blood changes rapidly in neutrophils and monocytes after ischemic stroke in humans: A microarray study', Journal of Cerebral Blood Flow and Metabolism, vol. 26, no. 8, pp. 1089-1102. https://doi.org/10.1038/sj.jcbfm.9600264
Tang, Yang ; Xu, Huichun ; Du, Xinli ; Lit, Lisa ; Walker, Wynn ; Lu, Aigang ; Ran, Ruiqiong ; Gregg, Jeffrey ; Reilly, Melinda ; Pancioli, Art ; Khoury, Jane C. ; Sauerbeck, Laura R. ; Carrozzella, Janice A. ; Spilker, Judith ; Clark, Joseph ; Wagner, Kenneth R. ; Jauch, Edward C. ; Chang, Dongwoo J. ; Verro, Piero ; Broderick, Joseph P. ; Sharp, Frank R. / Gene expression in blood changes rapidly in neutrophils and monocytes after ischemic stroke in humans : A microarray study. In: Journal of Cerebral Blood Flow and Metabolism. 2006 ; Vol. 26, No. 8. pp. 1089-1102.
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