Gene expression in a swine model of right ventricular hypertrophy: Intercellular adhesion molecule, vascular endothelial growth factor and plasminogen activators are upregulated during pressure overload

S. M. Carroll, L. E. Nimmo, Paul S Knoepfler, F. C. White, C. M. Bloor

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Abstract

We have investigated the molecular changes which occur during pressure overload hypertrophy of the RV in swine. Animals were banded on the pulmonary artery so that right ventricular pressure was increased two-fold. The heart was harvested at 3, 7, 24 and 72 h after surgery. Between 7 and 72 h there was evidence of muscle damage and inflammation. Northern blot experiments showed that pressure overload induced a transient increase in the expression of the immediate early genes and in the developmentally regulated atrial natriuretic factor and skeletal muscle α actin genes. Consistent with the histological observations of inflammation, increases in the expression of the gene for intercellular adhesion molecule, which encodes a protein involved in the binding of leukocytes by endothelial cells and myocytes, was observed between 3 and 24 h. In addition, the expression of vascular endothelial growth factor, a growth and permeability factor specific for endothelial cells was increased at 3 and 7 h of pressure overload. An increase in the expression of urokinase plasminogen activator and its inhibitors, plasminogen activator inhibitors I and II, was also observed between 3 and 24 h. This was associated with an increase in urokinase activity in the myocardial tissue. These results indicate that hypertrophy in a large mammal such as swine induces a program of gene expression similar to that previously described in rodents and suggests that up-regulation of a variety of other genes is an early response to pressure overload.

Original languageEnglish (US)
Pages (from-to)1427-1441
Number of pages15
JournalJournal of Molecular and Cellular Cardiology
Volume27
Issue number7
DOIs
StatePublished - 1995

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Right Ventricular Hypertrophy
Plasminogen Activators
Cell Adhesion Molecules
Vascular Endothelial Growth Factor A
Swine
Gene Expression
Plasminogen Inactivators
Pressure
Urokinase-Type Plasminogen Activator
Hypertrophy
Endothelial Cells
Inflammation
Immediate-Early Genes
Atrial Natriuretic Factor
Ventricular Pressure
Northern Blotting
Muscle Cells
Pulmonary Artery
Genes
Actins

Keywords

  • Hypertrophy
  • ICAM
  • Plasminogen activator
  • Swine
  • VEGF

ASJC Scopus subject areas

  • Molecular Biology
  • Cardiology and Cardiovascular Medicine

Cite this

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title = "Gene expression in a swine model of right ventricular hypertrophy: Intercellular adhesion molecule, vascular endothelial growth factor and plasminogen activators are upregulated during pressure overload",
abstract = "We have investigated the molecular changes which occur during pressure overload hypertrophy of the RV in swine. Animals were banded on the pulmonary artery so that right ventricular pressure was increased two-fold. The heart was harvested at 3, 7, 24 and 72 h after surgery. Between 7 and 72 h there was evidence of muscle damage and inflammation. Northern blot experiments showed that pressure overload induced a transient increase in the expression of the immediate early genes and in the developmentally regulated atrial natriuretic factor and skeletal muscle α actin genes. Consistent with the histological observations of inflammation, increases in the expression of the gene for intercellular adhesion molecule, which encodes a protein involved in the binding of leukocytes by endothelial cells and myocytes, was observed between 3 and 24 h. In addition, the expression of vascular endothelial growth factor, a growth and permeability factor specific for endothelial cells was increased at 3 and 7 h of pressure overload. An increase in the expression of urokinase plasminogen activator and its inhibitors, plasminogen activator inhibitors I and II, was also observed between 3 and 24 h. This was associated with an increase in urokinase activity in the myocardial tissue. These results indicate that hypertrophy in a large mammal such as swine induces a program of gene expression similar to that previously described in rodents and suggests that up-regulation of a variety of other genes is an early response to pressure overload.",
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AU - Nimmo, L. E.

AU - Knoepfler, Paul S

AU - White, F. C.

AU - Bloor, C. M.

PY - 1995

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