Gene expression changes in mouse brain after exposure to low-dose ionizing radiation

E. Yin, D. O. Nelson, Matthew A Coleman, L. E. Peterson, A. J. Wyrobek

Research output: Contribution to journalArticle

144 Citations (Scopus)

Abstract

Purpose: To characterize the cellular functions associated with the altered transcript profiles of mouse brain exposed to low-dose in vivo gamma-irradiation. Materials and methods: Cerebral RNA was isolated at 30 min and 4 h after whole-body irradiation at 0.1 or 2 Gy, hybridized to random oligonucleotide arrays, and evaluated for time and dose-response patterns by multifactorial analyses. Results: Brain irradiation modulated the expression patterns of 1574 genes, of which 855 showed more than 1.5-fold variation, about 30% of genes showed dose-dependent variations, including genes exclusively affected by 0.1 Gy. About 60% of genes showed time-dependent variation with more genes affected at 30 min than at 4 h. Early changes involved signal transduction, ion regulation and synaptic signalling. Later changes involved metabolic functions including myelin and protein synthesis. Low-dose radiation also modulated the expression of genes involved in stress response, cell-cycle control and DNA synthesis/repair. Conclusions: Doses of 0.1 Gy induced changes in gene expression that were qualitatively different from those at 2 Gy. The findings suggest that low-dose irradiation of the brain induces the expression of genes involved in protective and reparative functions, while down-modulating genes involved in neural signalling activity.

Original languageEnglish (US)
Pages (from-to)759-775
Number of pages17
JournalInternational Journal of Radiation Biology
Volume79
Issue number10
DOIs
StatePublished - Oct 2003
Externally publishedYes

Fingerprint

gene expression
Ionizing radiation
Ionizing Radiation
ionizing radiation
Gene expression
genes
Dosimetry
brain
mice
Brain
Genes
Gene Expression
dosage
irradiation
Irradiation
Myelin Proteins
Whole-Body Irradiation
myelin sheath
myelin
protein synthesis

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology
  • Nuclear Energy and Engineering
  • Radiation

Cite this

Gene expression changes in mouse brain after exposure to low-dose ionizing radiation. / Yin, E.; Nelson, D. O.; Coleman, Matthew A; Peterson, L. E.; Wyrobek, A. J.

In: International Journal of Radiation Biology, Vol. 79, No. 10, 10.2003, p. 759-775.

Research output: Contribution to journalArticle

Yin, E. ; Nelson, D. O. ; Coleman, Matthew A ; Peterson, L. E. ; Wyrobek, A. J. / Gene expression changes in mouse brain after exposure to low-dose ionizing radiation. In: International Journal of Radiation Biology. 2003 ; Vol. 79, No. 10. pp. 759-775.
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