Gene arrangement and RNA transcription of the BamHI fragments K and M2 within the non-oncogenic Marek's disease virus serotype 2 unique long genome region

Yoshinori Tsushima, Hyung Kwan Jang, Yoshihiro Izumiya, Jin Shun Cai, Kentaro Kato, Takayuki Miyazawa, Chieko Kai, Eiji Takahashi, Takeshi Mikami

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We determined the nucleotide sequence of a 6593 bp fragment of the Marek's disease virus serotype 2 (MDV2) unique long region located in the right part of genomic BamHI-M2 and the adjacent part of BamHI-K fragments. Within this region five complete open reading frames (ORFs) were identified whose deduced amino acid sequences exhibited homology to the UL53 (glycoprotein K), UL54 (immediate early regulatory protein ICP27), and UL55 gene products of herpes simplex virus type 1 (HSV-1). Homologue to the HSV-1 UL56 was not detected. However, we identified a gene between the MDV2 UL54 and UL55 genes with homology to the first ORF (ORF-1) of equine herpesvirus type 1 and corresponding gene identified in pseudorabies virus. Two adjacent ORFs contained in the BamHI-K fragment, ORF 873s and ORF 873, were found by computer analysis to have the properties of an intron encoding a glycoprotein: ORF 873s encodes a 84 amino acid polypeptide with a stretch of a hydrophobic signal sequence in the C-terminus, and ORF 873 encodes a 873 amino acid polypeptide with a transmembrane domain and putative three N-linked glycosylation sites. All the identified genes were confirmed to be transcribed with 3'-coterminal transcripts and/or a unique transcript in the virus-infected cells. Especially, 3.5 kb mRNA of ORF 873s and ORF 873 are transcribed from a potential promoter region of ORF 873s, and splice donor and acceptor sites are used to splice the mRNA after cleavage of a 113 bp-nucleotide sequence. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)101-110
Number of pages10
JournalVirus Research
Volume60
Issue number1
DOIs
StatePublished - Mar 1999
Externally publishedYes

Fingerprint

Gallid Herpesvirus 3
Gene Order
Open Reading Frames
Genome
RNA
RNA Splice Sites
Genes
Human Herpesvirus 1
Glycoproteins
Equid Herpesvirus 1
Immediate-Early Proteins
Suid Herpesvirus 1
Amino Acids
Amino Acid Sequence Homology
Messenger RNA
Peptides
Protein Sorting Signals

Keywords

  • MDV2 BamHI-K DNA
  • MDV2 BamHI-M2 DNA
  • Nucleotide sequence
  • Putative MDV specific glycoprotein

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology
  • Virology

Cite this

Gene arrangement and RNA transcription of the BamHI fragments K and M2 within the non-oncogenic Marek's disease virus serotype 2 unique long genome region. / Tsushima, Yoshinori; Jang, Hyung Kwan; Izumiya, Yoshihiro; Cai, Jin Shun; Kato, Kentaro; Miyazawa, Takayuki; Kai, Chieko; Takahashi, Eiji; Mikami, Takeshi.

In: Virus Research, Vol. 60, No. 1, 03.1999, p. 101-110.

Research output: Contribution to journalArticle

Tsushima, Yoshinori ; Jang, Hyung Kwan ; Izumiya, Yoshihiro ; Cai, Jin Shun ; Kato, Kentaro ; Miyazawa, Takayuki ; Kai, Chieko ; Takahashi, Eiji ; Mikami, Takeshi. / Gene arrangement and RNA transcription of the BamHI fragments K and M2 within the non-oncogenic Marek's disease virus serotype 2 unique long genome region. In: Virus Research. 1999 ; Vol. 60, No. 1. pp. 101-110.
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AU - Tsushima, Yoshinori

AU - Jang, Hyung Kwan

AU - Izumiya, Yoshihiro

AU - Cai, Jin Shun

AU - Kato, Kentaro

AU - Miyazawa, Takayuki

AU - Kai, Chieko

AU - Takahashi, Eiji

AU - Mikami, Takeshi

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N2 - We determined the nucleotide sequence of a 6593 bp fragment of the Marek's disease virus serotype 2 (MDV2) unique long region located in the right part of genomic BamHI-M2 and the adjacent part of BamHI-K fragments. Within this region five complete open reading frames (ORFs) were identified whose deduced amino acid sequences exhibited homology to the UL53 (glycoprotein K), UL54 (immediate early regulatory protein ICP27), and UL55 gene products of herpes simplex virus type 1 (HSV-1). Homologue to the HSV-1 UL56 was not detected. However, we identified a gene between the MDV2 UL54 and UL55 genes with homology to the first ORF (ORF-1) of equine herpesvirus type 1 and corresponding gene identified in pseudorabies virus. Two adjacent ORFs contained in the BamHI-K fragment, ORF 873s and ORF 873, were found by computer analysis to have the properties of an intron encoding a glycoprotein: ORF 873s encodes a 84 amino acid polypeptide with a stretch of a hydrophobic signal sequence in the C-terminus, and ORF 873 encodes a 873 amino acid polypeptide with a transmembrane domain and putative three N-linked glycosylation sites. All the identified genes were confirmed to be transcribed with 3'-coterminal transcripts and/or a unique transcript in the virus-infected cells. Especially, 3.5 kb mRNA of ORF 873s and ORF 873 are transcribed from a potential promoter region of ORF 873s, and splice donor and acceptor sites are used to splice the mRNA after cleavage of a 113 bp-nucleotide sequence. Copyright (C) 1999 Elsevier Science B.V.

AB - We determined the nucleotide sequence of a 6593 bp fragment of the Marek's disease virus serotype 2 (MDV2) unique long region located in the right part of genomic BamHI-M2 and the adjacent part of BamHI-K fragments. Within this region five complete open reading frames (ORFs) were identified whose deduced amino acid sequences exhibited homology to the UL53 (glycoprotein K), UL54 (immediate early regulatory protein ICP27), and UL55 gene products of herpes simplex virus type 1 (HSV-1). Homologue to the HSV-1 UL56 was not detected. However, we identified a gene between the MDV2 UL54 and UL55 genes with homology to the first ORF (ORF-1) of equine herpesvirus type 1 and corresponding gene identified in pseudorabies virus. Two adjacent ORFs contained in the BamHI-K fragment, ORF 873s and ORF 873, were found by computer analysis to have the properties of an intron encoding a glycoprotein: ORF 873s encodes a 84 amino acid polypeptide with a stretch of a hydrophobic signal sequence in the C-terminus, and ORF 873 encodes a 873 amino acid polypeptide with a transmembrane domain and putative three N-linked glycosylation sites. All the identified genes were confirmed to be transcribed with 3'-coterminal transcripts and/or a unique transcript in the virus-infected cells. Especially, 3.5 kb mRNA of ORF 873s and ORF 873 are transcribed from a potential promoter region of ORF 873s, and splice donor and acceptor sites are used to splice the mRNA after cleavage of a 113 bp-nucleotide sequence. Copyright (C) 1999 Elsevier Science B.V.

KW - MDV2 BamHI-K DNA

KW - MDV2 BamHI-M2 DNA

KW - Nucleotide sequence

KW - Putative MDV specific glycoprotein

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