Gender-Specific Gene Expression in Post-Mortem Human Brain: Localization to Sex Chromosomes

Marquis P. Vawter, Simon Evans, Prabhakara V Choudary, Hiroaki Tomita, Jim Meador-Woodruff, Margherita Molnar, Jun Li, Juan F. Lopez, Rick Myers, David Cox, Stanley J. Watson, Huda Akil, Edward G. Jones, William E. Bunney

Research output: Contribution to journalArticle

149 Citations (Scopus)

Abstract

Gender differences in brain development and in the prevalence of neuropsychiatric disorders such as depression have been reported. Gender differences in human brain might be related to patterns of gene expression. Microarray technology is one useful method for investigation of gene expression in brain. We investigated gene expression, cell types, and regional expression patterns of differentially expressed sex chromosome genes in brain. We profiled gene expression in male and female dorsolateral prefrontal cortex, anterior cingulate cortex, and cerebellum using the Affymetrix oligonudeotide microarray platform. Differentially expressed genes between males and females on the Y chromosome (DBY, SMCY, UTY, RPS4Y, and USP9Y) and X chromosome (XIST) were confirmed using real-time PCR measurements. In situ hybridization confirmed the differential expression of gender-specific genes and neuronal expression of XIST, RPS4Y, SMCY, and UTY in three brain regions examined. The XIST gene, which silences gene expression on regions of the X chromosome, is expressed in a subset of neurons. Since a subset of neurons express gender-specific genes, neural subpopulations may exhibit a subtle sexual dimorphism at the level of differences in gene regulation and function. The distinctive pattern of neuronal expression of XIST, RPS4Y, SMCY, and UTY and other sex chromosome genes in neuronal subpopulations may possibly contribute to gender differences in prevalence noted for some neuropsychiatric disorders. Studies of the protein expression of these sex-chromosome-linked genes in brain tissue are required to address the functional consequences of the observed gene expression differences.

Original languageEnglish (US)
Pages (from-to)373-384
Number of pages12
JournalNeuropsychopharmacology
Volume29
Issue number2
DOIs
StatePublished - Feb 2004

Fingerprint

Sex Chromosomes
Gene Expression
Brain
Genes
X Chromosome
Neurons
Y Chromosome
Gyrus Cinguli
Prefrontal Cortex
Sex Characteristics
Cerebellum
In Situ Hybridization
Real-Time Polymerase Chain Reaction
Technology

Keywords

  • DBY
  • RPS4Y
  • SMCY
  • USP9Y
  • UTY
  • VCX
  • VCY
  • XIST

ASJC Scopus subject areas

  • Pharmacology

Cite this

Vawter, M. P., Evans, S., Choudary, P. V., Tomita, H., Meador-Woodruff, J., Molnar, M., ... Bunney, W. E. (2004). Gender-Specific Gene Expression in Post-Mortem Human Brain: Localization to Sex Chromosomes. Neuropsychopharmacology, 29(2), 373-384. https://doi.org/10.1038/sj.npp.1300337

Gender-Specific Gene Expression in Post-Mortem Human Brain : Localization to Sex Chromosomes. / Vawter, Marquis P.; Evans, Simon; Choudary, Prabhakara V; Tomita, Hiroaki; Meador-Woodruff, Jim; Molnar, Margherita; Li, Jun; Lopez, Juan F.; Myers, Rick; Cox, David; Watson, Stanley J.; Akil, Huda; Jones, Edward G.; Bunney, William E.

In: Neuropsychopharmacology, Vol. 29, No. 2, 02.2004, p. 373-384.

Research output: Contribution to journalArticle

Vawter, MP, Evans, S, Choudary, PV, Tomita, H, Meador-Woodruff, J, Molnar, M, Li, J, Lopez, JF, Myers, R, Cox, D, Watson, SJ, Akil, H, Jones, EG & Bunney, WE 2004, 'Gender-Specific Gene Expression in Post-Mortem Human Brain: Localization to Sex Chromosomes', Neuropsychopharmacology, vol. 29, no. 2, pp. 373-384. https://doi.org/10.1038/sj.npp.1300337
Vawter MP, Evans S, Choudary PV, Tomita H, Meador-Woodruff J, Molnar M et al. Gender-Specific Gene Expression in Post-Mortem Human Brain: Localization to Sex Chromosomes. Neuropsychopharmacology. 2004 Feb;29(2):373-384. https://doi.org/10.1038/sj.npp.1300337
Vawter, Marquis P. ; Evans, Simon ; Choudary, Prabhakara V ; Tomita, Hiroaki ; Meador-Woodruff, Jim ; Molnar, Margherita ; Li, Jun ; Lopez, Juan F. ; Myers, Rick ; Cox, David ; Watson, Stanley J. ; Akil, Huda ; Jones, Edward G. ; Bunney, William E. / Gender-Specific Gene Expression in Post-Mortem Human Brain : Localization to Sex Chromosomes. In: Neuropsychopharmacology. 2004 ; Vol. 29, No. 2. pp. 373-384.
@article{d79a976fb8164f30a37389e837ff7279,
title = "Gender-Specific Gene Expression in Post-Mortem Human Brain: Localization to Sex Chromosomes",
abstract = "Gender differences in brain development and in the prevalence of neuropsychiatric disorders such as depression have been reported. Gender differences in human brain might be related to patterns of gene expression. Microarray technology is one useful method for investigation of gene expression in brain. We investigated gene expression, cell types, and regional expression patterns of differentially expressed sex chromosome genes in brain. We profiled gene expression in male and female dorsolateral prefrontal cortex, anterior cingulate cortex, and cerebellum using the Affymetrix oligonudeotide microarray platform. Differentially expressed genes between males and females on the Y chromosome (DBY, SMCY, UTY, RPS4Y, and USP9Y) and X chromosome (XIST) were confirmed using real-time PCR measurements. In situ hybridization confirmed the differential expression of gender-specific genes and neuronal expression of XIST, RPS4Y, SMCY, and UTY in three brain regions examined. The XIST gene, which silences gene expression on regions of the X chromosome, is expressed in a subset of neurons. Since a subset of neurons express gender-specific genes, neural subpopulations may exhibit a subtle sexual dimorphism at the level of differences in gene regulation and function. The distinctive pattern of neuronal expression of XIST, RPS4Y, SMCY, and UTY and other sex chromosome genes in neuronal subpopulations may possibly contribute to gender differences in prevalence noted for some neuropsychiatric disorders. Studies of the protein expression of these sex-chromosome-linked genes in brain tissue are required to address the functional consequences of the observed gene expression differences.",
keywords = "DBY, RPS4Y, SMCY, USP9Y, UTY, VCX, VCY, XIST",
author = "Vawter, {Marquis P.} and Simon Evans and Choudary, {Prabhakara V} and Hiroaki Tomita and Jim Meador-Woodruff and Margherita Molnar and Jun Li and Lopez, {Juan F.} and Rick Myers and David Cox and Watson, {Stanley J.} and Huda Akil and Jones, {Edward G.} and Bunney, {William E.}",
year = "2004",
month = "2",
doi = "10.1038/sj.npp.1300337",
language = "English (US)",
volume = "29",
pages = "373--384",
journal = "Neuropsychopharmacology",
issn = "0893-133X",
publisher = "Nature Publishing Group",
number = "2",

}

TY - JOUR

T1 - Gender-Specific Gene Expression in Post-Mortem Human Brain

T2 - Localization to Sex Chromosomes

AU - Vawter, Marquis P.

AU - Evans, Simon

AU - Choudary, Prabhakara V

AU - Tomita, Hiroaki

AU - Meador-Woodruff, Jim

AU - Molnar, Margherita

AU - Li, Jun

AU - Lopez, Juan F.

AU - Myers, Rick

AU - Cox, David

AU - Watson, Stanley J.

AU - Akil, Huda

AU - Jones, Edward G.

AU - Bunney, William E.

PY - 2004/2

Y1 - 2004/2

N2 - Gender differences in brain development and in the prevalence of neuropsychiatric disorders such as depression have been reported. Gender differences in human brain might be related to patterns of gene expression. Microarray technology is one useful method for investigation of gene expression in brain. We investigated gene expression, cell types, and regional expression patterns of differentially expressed sex chromosome genes in brain. We profiled gene expression in male and female dorsolateral prefrontal cortex, anterior cingulate cortex, and cerebellum using the Affymetrix oligonudeotide microarray platform. Differentially expressed genes between males and females on the Y chromosome (DBY, SMCY, UTY, RPS4Y, and USP9Y) and X chromosome (XIST) were confirmed using real-time PCR measurements. In situ hybridization confirmed the differential expression of gender-specific genes and neuronal expression of XIST, RPS4Y, SMCY, and UTY in three brain regions examined. The XIST gene, which silences gene expression on regions of the X chromosome, is expressed in a subset of neurons. Since a subset of neurons express gender-specific genes, neural subpopulations may exhibit a subtle sexual dimorphism at the level of differences in gene regulation and function. The distinctive pattern of neuronal expression of XIST, RPS4Y, SMCY, and UTY and other sex chromosome genes in neuronal subpopulations may possibly contribute to gender differences in prevalence noted for some neuropsychiatric disorders. Studies of the protein expression of these sex-chromosome-linked genes in brain tissue are required to address the functional consequences of the observed gene expression differences.

AB - Gender differences in brain development and in the prevalence of neuropsychiatric disorders such as depression have been reported. Gender differences in human brain might be related to patterns of gene expression. Microarray technology is one useful method for investigation of gene expression in brain. We investigated gene expression, cell types, and regional expression patterns of differentially expressed sex chromosome genes in brain. We profiled gene expression in male and female dorsolateral prefrontal cortex, anterior cingulate cortex, and cerebellum using the Affymetrix oligonudeotide microarray platform. Differentially expressed genes between males and females on the Y chromosome (DBY, SMCY, UTY, RPS4Y, and USP9Y) and X chromosome (XIST) were confirmed using real-time PCR measurements. In situ hybridization confirmed the differential expression of gender-specific genes and neuronal expression of XIST, RPS4Y, SMCY, and UTY in three brain regions examined. The XIST gene, which silences gene expression on regions of the X chromosome, is expressed in a subset of neurons. Since a subset of neurons express gender-specific genes, neural subpopulations may exhibit a subtle sexual dimorphism at the level of differences in gene regulation and function. The distinctive pattern of neuronal expression of XIST, RPS4Y, SMCY, and UTY and other sex chromosome genes in neuronal subpopulations may possibly contribute to gender differences in prevalence noted for some neuropsychiatric disorders. Studies of the protein expression of these sex-chromosome-linked genes in brain tissue are required to address the functional consequences of the observed gene expression differences.

KW - DBY

KW - RPS4Y

KW - SMCY

KW - USP9Y

KW - UTY

KW - VCX

KW - VCY

KW - XIST

UR - http://www.scopus.com/inward/record.url?scp=10744226630&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=10744226630&partnerID=8YFLogxK

U2 - 10.1038/sj.npp.1300337

DO - 10.1038/sj.npp.1300337

M3 - Article

C2 - 14583743

AN - SCOPUS:10744226630

VL - 29

SP - 373

EP - 384

JO - Neuropsychopharmacology

JF - Neuropsychopharmacology

SN - 0893-133X

IS - 2

ER -