Gender Differences in Bile Acids and Microbiota in Relationship with Gender Dissimilarity in Steatosis Induced by Diet and FXR Inactivation

Lili Sheng, Prasant Kumar Jena, Hui Xin Liu, Karen M. Kalanetra, Frank J. Gonzalez, Samuel W. French, Viswanathan V Krishnan, David A. Mills, Yu-Jui Yvonne Wan

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

This study aims to uncover how specific bacteria and bile acids (BAs) contribute to steatosis induced by diet and farnesoid X receptor (FXR) deficiency in both genders. A control diet (CD) and Western diet (WD), which contains high fat and carbohydrate, were used to feed wild type (WT) and FXR knockout (KO) mice followed by phenotyping characterization as well as BA and microbiota profiling. Our data revealed that male WD-fed FXR KO mice had the most severe steatosis and highest hepatic and serum lipids as well as insulin resistance among the eight studied groups. Gender differences in WD-induced steatosis, insulin sensitivity, and predicted microbiota functions were all FXR-dependent. FXR deficiency enriched Desulfovibrionaceae, Deferribacteraceae, and Helicobacteraceae, which were accompanied by increased hepatic taurine-conjugated cholic acid and β-muricholic acid as well as hepatic and serum lipids. Additionally, distinct microbiota profiles were found in WD-fed WT mice harboring simple steatosis and CD-fed FXR KO mice, in which the steatosis had a potential to develop into liver cancer. Together, the presented data revealed FXR-dependent concomitant relationships between gut microbiota, BAs, and metabolic diseases in both genders. Gender differences in BAs and microbiota may account for gender dissimilarity in metabolism and metabolic diseases.

Original languageEnglish (US)
Article number1748
JournalScientific Reports
Volume7
Issue number1
DOIs
StatePublished - Dec 1 2017

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X Chromosome Inactivation
Microbiota
Bile Acids and Salts
Knockout Mice
Diet
Metabolic Diseases
Insulin Resistance
Liver
Lipids
Cholic Acid
Taurine
Liver Neoplasms
Serum
Fats
Carbohydrates
Bacteria
Western Diet

ASJC Scopus subject areas

  • General

Cite this

Gender Differences in Bile Acids and Microbiota in Relationship with Gender Dissimilarity in Steatosis Induced by Diet and FXR Inactivation. / Sheng, Lili; Jena, Prasant Kumar; Liu, Hui Xin; Kalanetra, Karen M.; Gonzalez, Frank J.; French, Samuel W.; Krishnan, Viswanathan V; Mills, David A.; Wan, Yu-Jui Yvonne.

In: Scientific Reports, Vol. 7, No. 1, 1748, 01.12.2017.

Research output: Contribution to journalArticle

Sheng, Lili ; Jena, Prasant Kumar ; Liu, Hui Xin ; Kalanetra, Karen M. ; Gonzalez, Frank J. ; French, Samuel W. ; Krishnan, Viswanathan V ; Mills, David A. ; Wan, Yu-Jui Yvonne. / Gender Differences in Bile Acids and Microbiota in Relationship with Gender Dissimilarity in Steatosis Induced by Diet and FXR Inactivation. In: Scientific Reports. 2017 ; Vol. 7, No. 1.
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