Abstract
The purpose of this in vitro study was to compare the degradation of gemcitabine (2', 2'-difluorodeoxycytidine, dFdC), in Fresh Whole Blood (FWB) from humans, dogs, cats, and horses. A better understanding of the comparative degradation of gemcitabine may aid in the optimal design of therapeutic regimens in veterinary species. Fresh whole blood from humans, dogs, cats, and horses was spiked with dFdC and plasma was analyzed for dFdC and 2', 2'-difluorodeoxyuridine (dFdU) by high performance liquid chromatography. In these species, there was an initial rapid degradation of dFdC with a concomitant proportional increase in dFdU. Degradation of gemcitabine appeared similar in humans, dogs, and horses (p>0.05) whereas metabolism was slower in the cat than human (p=0.014), dog (p=0.010), or horse (p=0.0015). Based on these in vitro findings, dosing schemes for humans, dogs, and horses may be similar. In contrast, gemcitabine degradation occurred more slowly in the cat; this difference may dictate a different dosing scheme for optimal response in this species.
| Original language | English (US) |
|---|---|
| Article number | 119 |
| Journal | Journal of Veterinary Science and Technology |
| Volume | 3 |
| Issue number | 5 |
| DOIs | |
| State | Published - 2012 |
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Keywords
- Canine
- Equinel
- Feline
- Gemcitabine
- Plasma catabolism
ASJC Scopus subject areas
- veterinary(all)
Cite this
Gemcitabine degradation in whole blood from humans, dogs, cats, and horses. / O'Brien, Danielle R.; Guerrero, Teri A.; Frazier, Sara Allstadt; Rebhun, Robert B; Skorupski, Katherine A; Rodriguez, Carlos O.
In: Journal of Veterinary Science and Technology, Vol. 3, No. 5, 119, 2012.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Gemcitabine degradation in whole blood from humans, dogs, cats, and horses
AU - O'Brien, Danielle R.
AU - Guerrero, Teri A.
AU - Frazier, Sara Allstadt
AU - Rebhun, Robert B
AU - Skorupski, Katherine A
AU - Rodriguez, Carlos O.
PY - 2012
Y1 - 2012
N2 - The purpose of this in vitro study was to compare the degradation of gemcitabine (2', 2'-difluorodeoxycytidine, dFdC), in Fresh Whole Blood (FWB) from humans, dogs, cats, and horses. A better understanding of the comparative degradation of gemcitabine may aid in the optimal design of therapeutic regimens in veterinary species. Fresh whole blood from humans, dogs, cats, and horses was spiked with dFdC and plasma was analyzed for dFdC and 2', 2'-difluorodeoxyuridine (dFdU) by high performance liquid chromatography. In these species, there was an initial rapid degradation of dFdC with a concomitant proportional increase in dFdU. Degradation of gemcitabine appeared similar in humans, dogs, and horses (p>0.05) whereas metabolism was slower in the cat than human (p=0.014), dog (p=0.010), or horse (p=0.0015). Based on these in vitro findings, dosing schemes for humans, dogs, and horses may be similar. In contrast, gemcitabine degradation occurred more slowly in the cat; this difference may dictate a different dosing scheme for optimal response in this species.
AB - The purpose of this in vitro study was to compare the degradation of gemcitabine (2', 2'-difluorodeoxycytidine, dFdC), in Fresh Whole Blood (FWB) from humans, dogs, cats, and horses. A better understanding of the comparative degradation of gemcitabine may aid in the optimal design of therapeutic regimens in veterinary species. Fresh whole blood from humans, dogs, cats, and horses was spiked with dFdC and plasma was analyzed for dFdC and 2', 2'-difluorodeoxyuridine (dFdU) by high performance liquid chromatography. In these species, there was an initial rapid degradation of dFdC with a concomitant proportional increase in dFdU. Degradation of gemcitabine appeared similar in humans, dogs, and horses (p>0.05) whereas metabolism was slower in the cat than human (p=0.014), dog (p=0.010), or horse (p=0.0015). Based on these in vitro findings, dosing schemes for humans, dogs, and horses may be similar. In contrast, gemcitabine degradation occurred more slowly in the cat; this difference may dictate a different dosing scheme for optimal response in this species.
KW - Canine
KW - Equinel
KW - Feline
KW - Gemcitabine
KW - Plasma catabolism
UR - http://www.scopus.com/inward/record.url?scp=84881483007&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84881483007&partnerID=8YFLogxK
U2 - 10.4172/2157-7579.1000119
DO - 10.4172/2157-7579.1000119
M3 - Article
AN - SCOPUS:84881483007
VL - 3
JO - Journal of Veterinary Science and Technology
JF - Journal of Veterinary Science and Technology
SN - 2157-7579
IS - 5
M1 - 119
ER -