Gelatinase activity in keloids and hypertrophic scars

A. N. Neely, C. E. Clendening, J. Gardner, David G Greenhalgh, G. D. Warden

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

Keloids and hypertrophic scars are characterized by excessive deposition of collagen, which may result from insufficient protein degradation. Little is known about the levels of two gelatinases, matrix metalloproteinase-2 (72 kD type IV collagenase) and matrix metalloproteinase-9 (matrix metalloproteinase-9; 92 kD type IV collagenase) in these abnormal scars. The purpose of this study was to determine levels of these proteinases in tissue from hypertrophic scars, keloids, and donor skin. Ten hypertrophic scar samples, 9 keloid samples, and 10 donor skin samples were frozen, pulverized, homogenized, clarified by centrifugation, and analyzed for matrix metalloproteinases by quantitative zymography. Identity of matrix metalloproteinases was determined using a conditioned media reference standard, molecular weight ladders, and Western blotting. Levels of matrix metalloproteinase-9 activity were very low or undetectable in all samples. However, matrix metalloproteinase-2 activity was significantly elevated in keloids and hypertrophic scars vs. donor samples: 2.6 and 3.9-fold increases for latent matrix metalloproteinase-2, 7.8 and 6.9-fold increases for active matrix metalloproteinase-2, respectively. We conclude that little matrix metalloproteinase-9 activity (the gelatinase involved in early tissue repair) is present in keloids and hypertrophic scars, while matrix metalloproteinase- 2 activity (the gelatinase involved in prolonged tissue remodeling) is present in donor skin and is significantly increased in hypertrophic scars and keloids.

Original languageEnglish (US)
Pages (from-to)166-171
Number of pages6
JournalWound Repair and Regeneration
Volume7
Issue number3
DOIs
StatePublished - 1999
Externally publishedYes

Fingerprint

Hypertrophic Cicatrix
Keloid
Gelatinases
Matrix Metalloproteinase 2
Matrix Metalloproteinase 9
Collagenases
Matrix Metalloproteinases
Skin
Conditioned Culture Medium
Centrifugation
Proteolysis
Cicatrix
Peptide Hydrolases
Collagen
Molecular Weight
Western Blotting

ASJC Scopus subject areas

  • Dermatology
  • Surgery

Cite this

Gelatinase activity in keloids and hypertrophic scars. / Neely, A. N.; Clendening, C. E.; Gardner, J.; Greenhalgh, David G; Warden, G. D.

In: Wound Repair and Regeneration, Vol. 7, No. 3, 1999, p. 166-171.

Research output: Contribution to journalArticle

Neely, A. N. ; Clendening, C. E. ; Gardner, J. ; Greenhalgh, David G ; Warden, G. D. / Gelatinase activity in keloids and hypertrophic scars. In: Wound Repair and Regeneration. 1999 ; Vol. 7, No. 3. pp. 166-171.
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