Gelatin-sealed polyester resists Staphylococcus epidermidis biofilm infection

Michael Farooq, Julie Freischlag, Holly Kelly, Gary Seabrook, Robert Cambria, Jonathan Towne

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Background. The purpose of this study was to show that gelatin- impregnated polyester grafts inhibit Staphylococcus epidermidis biofilm infection in a canine model of aortic graft interposition. A clinically native species and two engineered strains, which differed in slime and adhesin antigen components, were compared to determine differential gelatin and slime interactions. Methods. In vitro bacterial graft colonization was validated by immersion of graft segments in inoculating solutions (106 colony forming units/ml) of a clinically native species RP62A and two genetically engineered S. epidermidis species, M187sn3 (SN3: slime and adhesin negative) or M187sp11 (SP11: slime and adhesin positive), for 18 h at 23°C. The grafts were washed, sonicated, and cultured to assess in vitro bacterial graft adherence. Grafts similarly inoculated were placed as aortic interposition grafts in dogs. Three sterile grafts were implanted as controls. Grafts were excised after 6 weeks and cultured for bacterial growth as in the in vitro study. Infection was defined by a positive culture in the excised grafts. Data were analyzed with nonparametric statistical methods. Results. In vitro bacterial graft adherence in colony forming units per milliliter was similar at 18 h post- sonication for RP62A (8 x 104 ± 1 x 104), SN3 (7 x 104 ± 2 x 104), and SP11 (6 x 104 ± 2 x 104) (P = NS). Only one of five grafts inoculated with RP62A was culture positive after 6 weeks. No grafts inoculated with the engineered strains SN3 or SP11 were culture positive after explanation. Conclusion. In vitro bacterial inoculation of gelatin-impregnated polyester was similar among the species and not dependent upon the presence of slime and adhesin components. Gelatin- impregnated polyester grafts demonstrated in vivo resistance to coagulase- negative staphylococcal biofilm infection.

Original languageEnglish (US)
Pages (from-to)57-61
Number of pages5
JournalJournal of Surgical Research
Volume87
Issue number1
DOIs
StatePublished - Nov 1999
Externally publishedYes

Fingerprint

Polyesters
Staphylococcus epidermidis
Gelatin
Biofilms
Transplants
Infection
Stem Cells
Staphylococcal Infections
Sonication
Coagulase
Immersion

Keywords

  • Biofilm infection
  • Gelatin
  • Polyester graft
  • Staphylococcus epidermidis

ASJC Scopus subject areas

  • Surgery

Cite this

Farooq, M., Freischlag, J., Kelly, H., Seabrook, G., Cambria, R., & Towne, J. (1999). Gelatin-sealed polyester resists Staphylococcus epidermidis biofilm infection. Journal of Surgical Research, 87(1), 57-61. https://doi.org/10.1006/jsre.1999.5729

Gelatin-sealed polyester resists Staphylococcus epidermidis biofilm infection. / Farooq, Michael; Freischlag, Julie; Kelly, Holly; Seabrook, Gary; Cambria, Robert; Towne, Jonathan.

In: Journal of Surgical Research, Vol. 87, No. 1, 11.1999, p. 57-61.

Research output: Contribution to journalArticle

Farooq, M, Freischlag, J, Kelly, H, Seabrook, G, Cambria, R & Towne, J 1999, 'Gelatin-sealed polyester resists Staphylococcus epidermidis biofilm infection', Journal of Surgical Research, vol. 87, no. 1, pp. 57-61. https://doi.org/10.1006/jsre.1999.5729
Farooq, Michael ; Freischlag, Julie ; Kelly, Holly ; Seabrook, Gary ; Cambria, Robert ; Towne, Jonathan. / Gelatin-sealed polyester resists Staphylococcus epidermidis biofilm infection. In: Journal of Surgical Research. 1999 ; Vol. 87, No. 1. pp. 57-61.
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abstract = "Background. The purpose of this study was to show that gelatin- impregnated polyester grafts inhibit Staphylococcus epidermidis biofilm infection in a canine model of aortic graft interposition. A clinically native species and two engineered strains, which differed in slime and adhesin antigen components, were compared to determine differential gelatin and slime interactions. Methods. In vitro bacterial graft colonization was validated by immersion of graft segments in inoculating solutions (106 colony forming units/ml) of a clinically native species RP62A and two genetically engineered S. epidermidis species, M187sn3 (SN3: slime and adhesin negative) or M187sp11 (SP11: slime and adhesin positive), for 18 h at 23°C. The grafts were washed, sonicated, and cultured to assess in vitro bacterial graft adherence. Grafts similarly inoculated were placed as aortic interposition grafts in dogs. Three sterile grafts were implanted as controls. Grafts were excised after 6 weeks and cultured for bacterial growth as in the in vitro study. Infection was defined by a positive culture in the excised grafts. Data were analyzed with nonparametric statistical methods. Results. In vitro bacterial graft adherence in colony forming units per milliliter was similar at 18 h post- sonication for RP62A (8 x 104 ± 1 x 104), SN3 (7 x 104 ± 2 x 104), and SP11 (6 x 104 ± 2 x 104) (P = NS). Only one of five grafts inoculated with RP62A was culture positive after 6 weeks. No grafts inoculated with the engineered strains SN3 or SP11 were culture positive after explanation. Conclusion. In vitro bacterial inoculation of gelatin-impregnated polyester was similar among the species and not dependent upon the presence of slime and adhesin components. Gelatin- impregnated polyester grafts demonstrated in vivo resistance to coagulase- negative staphylococcal biofilm infection.",
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AU - Towne, Jonathan

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N2 - Background. The purpose of this study was to show that gelatin- impregnated polyester grafts inhibit Staphylococcus epidermidis biofilm infection in a canine model of aortic graft interposition. A clinically native species and two engineered strains, which differed in slime and adhesin antigen components, were compared to determine differential gelatin and slime interactions. Methods. In vitro bacterial graft colonization was validated by immersion of graft segments in inoculating solutions (106 colony forming units/ml) of a clinically native species RP62A and two genetically engineered S. epidermidis species, M187sn3 (SN3: slime and adhesin negative) or M187sp11 (SP11: slime and adhesin positive), for 18 h at 23°C. The grafts were washed, sonicated, and cultured to assess in vitro bacterial graft adherence. Grafts similarly inoculated were placed as aortic interposition grafts in dogs. Three sterile grafts were implanted as controls. Grafts were excised after 6 weeks and cultured for bacterial growth as in the in vitro study. Infection was defined by a positive culture in the excised grafts. Data were analyzed with nonparametric statistical methods. Results. In vitro bacterial graft adherence in colony forming units per milliliter was similar at 18 h post- sonication for RP62A (8 x 104 ± 1 x 104), SN3 (7 x 104 ± 2 x 104), and SP11 (6 x 104 ± 2 x 104) (P = NS). Only one of five grafts inoculated with RP62A was culture positive after 6 weeks. No grafts inoculated with the engineered strains SN3 or SP11 were culture positive after explanation. Conclusion. In vitro bacterial inoculation of gelatin-impregnated polyester was similar among the species and not dependent upon the presence of slime and adhesin components. Gelatin- impregnated polyester grafts demonstrated in vivo resistance to coagulase- negative staphylococcal biofilm infection.

AB - Background. The purpose of this study was to show that gelatin- impregnated polyester grafts inhibit Staphylococcus epidermidis biofilm infection in a canine model of aortic graft interposition. A clinically native species and two engineered strains, which differed in slime and adhesin antigen components, were compared to determine differential gelatin and slime interactions. Methods. In vitro bacterial graft colonization was validated by immersion of graft segments in inoculating solutions (106 colony forming units/ml) of a clinically native species RP62A and two genetically engineered S. epidermidis species, M187sn3 (SN3: slime and adhesin negative) or M187sp11 (SP11: slime and adhesin positive), for 18 h at 23°C. The grafts were washed, sonicated, and cultured to assess in vitro bacterial graft adherence. Grafts similarly inoculated were placed as aortic interposition grafts in dogs. Three sterile grafts were implanted as controls. Grafts were excised after 6 weeks and cultured for bacterial growth as in the in vitro study. Infection was defined by a positive culture in the excised grafts. Data were analyzed with nonparametric statistical methods. Results. In vitro bacterial graft adherence in colony forming units per milliliter was similar at 18 h post- sonication for RP62A (8 x 104 ± 1 x 104), SN3 (7 x 104 ± 2 x 104), and SP11 (6 x 104 ± 2 x 104) (P = NS). Only one of five grafts inoculated with RP62A was culture positive after 6 weeks. No grafts inoculated with the engineered strains SN3 or SP11 were culture positive after explanation. Conclusion. In vitro bacterial inoculation of gelatin-impregnated polyester was similar among the species and not dependent upon the presence of slime and adhesin components. Gelatin- impregnated polyester grafts demonstrated in vivo resistance to coagulase- negative staphylococcal biofilm infection.

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