Abstract
An increase in melting temperature for DNA:DNA duplexes had been observed previously (Zhu et al. Antisense Res. Dev. 3:349-356, 1993) when an oligo(δ)ornithine moiety was covalently appended to a short oligodeoxynucleotide. We now report the analysis of duplex formation by electrophoretic gel shift analysis. In the particular example studied, an increase in Tm of 4°C was found to correspond to about a fivefold increase in binding constant. A similar enhancement by the appended cationic peptide was observed when the target strand was RNA. The use of a competitive assay format for avoidance of adsorptive loss at low concentrations (<10-7 M) of the oligonucleotide-oligo(δ)ornithine conjugate is presented.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 69-74 |
| Number of pages | 6 |
| Journal | Antisense and Nucleic Acid Drug Development |
| Volume | 6 |
| Issue number | 1 |
| State | Published - Mar 1996 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Genetics
- Pharmacology
Cite this
Gel shift assay : Demonstration of enhanced binding of oligo(δ)-L-ornithine-oligodeoxynucleotide conjugates to complementary DNA and RNA. / Zhu, Tianmin; Pooyan, Shahriar; Wei, Ziping; Leibowitz, Michael J; Stein, Stanley.
In: Antisense and Nucleic Acid Drug Development, Vol. 6, No. 1, 03.1996, p. 69-74.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Gel shift assay
T2 - Demonstration of enhanced binding of oligo(δ)-L-ornithine-oligodeoxynucleotide conjugates to complementary DNA and RNA
AU - Zhu, Tianmin
AU - Pooyan, Shahriar
AU - Wei, Ziping
AU - Leibowitz, Michael J
AU - Stein, Stanley
PY - 1996/3
Y1 - 1996/3
N2 - An increase in melting temperature for DNA:DNA duplexes had been observed previously (Zhu et al. Antisense Res. Dev. 3:349-356, 1993) when an oligo(δ)ornithine moiety was covalently appended to a short oligodeoxynucleotide. We now report the analysis of duplex formation by electrophoretic gel shift analysis. In the particular example studied, an increase in Tm of 4°C was found to correspond to about a fivefold increase in binding constant. A similar enhancement by the appended cationic peptide was observed when the target strand was RNA. The use of a competitive assay format for avoidance of adsorptive loss at low concentrations (<10-7 M) of the oligonucleotide-oligo(δ)ornithine conjugate is presented.
AB - An increase in melting temperature for DNA:DNA duplexes had been observed previously (Zhu et al. Antisense Res. Dev. 3:349-356, 1993) when an oligo(δ)ornithine moiety was covalently appended to a short oligodeoxynucleotide. We now report the analysis of duplex formation by electrophoretic gel shift analysis. In the particular example studied, an increase in Tm of 4°C was found to correspond to about a fivefold increase in binding constant. A similar enhancement by the appended cationic peptide was observed when the target strand was RNA. The use of a competitive assay format for avoidance of adsorptive loss at low concentrations (<10-7 M) of the oligonucleotide-oligo(δ)ornithine conjugate is presented.
UR - http://www.scopus.com/inward/record.url?scp=0029975621&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029975621&partnerID=8YFLogxK
M3 - Article
C2 - 8783798
AN - SCOPUS:0029975621
VL - 6
SP - 69
EP - 74
JO - Nucleic Acid Therapeutics
JF - Nucleic Acid Therapeutics
SN - 2159-3337
IS - 1
ER -