Abstract
An increase in melting temperature for DNA:DNA duplexes had been observed previously (Zhu et al. Antisense Res. Dev. 3:349-356, 1993) when an oligo(δ)ornithine moiety was covalently appended to a short oligodeoxynucleotide. We now report the analysis of duplex formation by electrophoretic gel shift analysis. In the particular example studied, an increase in Tm of 4°C was found to correspond to about a fivefold increase in binding constant. A similar enhancement by the appended cationic peptide was observed when the target strand was RNA. The use of a competitive assay format for avoidance of adsorptive loss at low concentrations (<10-7 M) of the oligonucleotide-oligo(δ)ornithine conjugate is presented.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 69-74 |
| Number of pages | 6 |
| Journal | Antisense and Nucleic Acid Drug Development |
| Volume | 6 |
| Issue number | 1 |
| State | Published - Mar 1996 |
| Externally published | Yes |
ASJC Scopus subject areas
- Genetics
- Pharmacology
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Zhu, T., Pooyan, S., Wei, Z., Leibowitz, M. J., & Stein, S. (1996). Gel shift assay: Demonstration of enhanced binding of oligo(δ)-L-ornithine-oligodeoxynucleotide conjugates to complementary DNA and RNA. Antisense and Nucleic Acid Drug Development, 6(1), 69-74.