Gating charge interactions with the S1 segment during activation of a Na + channel voltage sensor

Paul G. DeCaen, Vladimir Yarov-Yarovoy, Todd Scheuer, William A. Catterall

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Voltage-gated Na + channels initiate action potentials during electrical signaling in excitable cells. Opening and closing of the pore of voltage-gated ion channels are mechanically linked to voltagedriven outward movement of the positively charged S4 transmembrane segment in their voltage sensors. Disulfide locking of cysteine residues substituted for the outermost T0 and R1 gating-charge positions and a conserved negative charge (E43) at the extracellular end of the S1 segment of the bacterial Na + channel NaChBac detects molecular interactions that stabilize the resting state of the voltage sensor and define its conformation. Upon depolarization, the more inward gating charges R2 and R3 engage in these molecular interactions as the S4 segment moves outward to its intermediate and activated states. The R4 gating charge does not disulfide-lock with E43, suggesting an outer limit to its transmembrane movement. These molecular interactions reveal how the S4 gating charges are stabilized in the resting state and how their outward movement is catalyzed by interaction with negatively charged residues to effect pore opening and initiate electrical signaling.

Original languageEnglish (US)
Pages (from-to)18825-18830
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume108
Issue number46
DOIs
StatePublished - Nov 15 2011

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Disulfides
Ion Channels
Action Potentials
Cysteine

Keywords

  • Sliding helix
  • Voltage-dependent gating

ASJC Scopus subject areas

  • General

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Gating charge interactions with the S1 segment during activation of a Na + channel voltage sensor. / DeCaen, Paul G.; Yarov-Yarovoy, Vladimir; Scheuer, Todd; Catterall, William A.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, No. 46, 15.11.2011, p. 18825-18830.

Research output: Contribution to journalArticle

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