Abstract
The gas-phase hydrogen/deuterium (HID) exchange kinetics of several protonated amino acids and dipeptides under a background pressure of CH 3OD were determined in an external source Fourier transform mass spectrometer. H/D exchange reactions occur even when the gas-phase basicity of the compound is significantly larger (> 20 kcal/mol) than methanol. In addition; greater deuterium incorporation is observed for compounds that have multiple sites of similar basicities. A mechanism is proposed that involves a structurally specific intermediate with extensive interaction between the protonated compound and methanol.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 623-631 |
| Number of pages | 9 |
| Journal | Journal of the American Society for Mass Spectrometry |
| Volume | 5 |
| Issue number | 7 |
| DOIs | |
| State | Published - Jul 1994 |
Fingerprint
ASJC Scopus subject areas
- Spectroscopy
- Structural Biology
Cite this
Gas-phase hydrogen/deuterium exchange as a molecular probe for the interaction of methanol and protonated peptides. / Gard, Eric; Green, M. Kirk; Bregar, Jennifer; Lebrilla, Carlito B.
In: Journal of the American Society for Mass Spectrometry, Vol. 5, No. 7, 07.1994, p. 623-631.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Gas-phase hydrogen/deuterium exchange as a molecular probe for the interaction of methanol and protonated peptides
AU - Gard, Eric
AU - Green, M. Kirk
AU - Bregar, Jennifer
AU - Lebrilla, Carlito B
PY - 1994/7
Y1 - 1994/7
N2 - The gas-phase hydrogen/deuterium (HID) exchange kinetics of several protonated amino acids and dipeptides under a background pressure of CH 3OD were determined in an external source Fourier transform mass spectrometer. H/D exchange reactions occur even when the gas-phase basicity of the compound is significantly larger (> 20 kcal/mol) than methanol. In addition; greater deuterium incorporation is observed for compounds that have multiple sites of similar basicities. A mechanism is proposed that involves a structurally specific intermediate with extensive interaction between the protonated compound and methanol.
AB - The gas-phase hydrogen/deuterium (HID) exchange kinetics of several protonated amino acids and dipeptides under a background pressure of CH 3OD were determined in an external source Fourier transform mass spectrometer. H/D exchange reactions occur even when the gas-phase basicity of the compound is significantly larger (> 20 kcal/mol) than methanol. In addition; greater deuterium incorporation is observed for compounds that have multiple sites of similar basicities. A mechanism is proposed that involves a structurally specific intermediate with extensive interaction between the protonated compound and methanol.
UR - http://www.scopus.com/inward/record.url?scp=0008153962&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0008153962&partnerID=8YFLogxK
U2 - 10.1016/1044-0305(94)85003-8
DO - 10.1016/1044-0305(94)85003-8
M3 - Article
C2 - 24221964
AN - SCOPUS:0008153962
VL - 5
SP - 623
EP - 631
JO - Journal of the American Society for Mass Spectrometry
JF - Journal of the American Society for Mass Spectrometry
SN - 1044-0305
IS - 7
ER -