Gas-phase hydrogen/deuterium exchange as a molecular probe for the interaction of methanol and protonated peptides

Eric Gard, M. Kirk Green, Jennifer Bregar, Carlito B Lebrilla

Research output: Contribution to journalArticle

112 Scopus citations

Abstract

The gas-phase hydrogen/deuterium (HID) exchange kinetics of several protonated amino acids and dipeptides under a background pressure of CH 3OD were determined in an external source Fourier transform mass spectrometer. H/D exchange reactions occur even when the gas-phase basicity of the compound is significantly larger (> 20 kcal/mol) than methanol. In addition; greater deuterium incorporation is observed for compounds that have multiple sites of similar basicities. A mechanism is proposed that involves a structurally specific intermediate with extensive interaction between the protonated compound and methanol.

Original languageEnglish (US)
Pages (from-to)623-631
Number of pages9
JournalJournal of the American Society for Mass Spectrometry
Volume5
Issue number7
DOIs
StatePublished - Jul 1994

ASJC Scopus subject areas

  • Spectroscopy
  • Structural Biology

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