Garlic (Allium sativum) extract inhibits lipopolysaccharide-induced toll-like receptor 4 dimerization

Hyung Sun Youn, Hyo Jin Lim, Hwa Jin Lee, Daniel Hwang, Mihi Yang, Raok Jeon, Jae Ha Ryu

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Garlic has long been used as a folk medicine. Numerous studies have demonstrated that a garlic extract and its sulfur-containing compounds inhibited nuclear factor kappa B (NF-κB) activation induced by various receptor agonists including lipopolysaccharide (LPS). Toll-like receptors (TLRs) play a key role in sensing diverse microbial products and inducing innate immune responses. The dimerization of TLR4 is required for the activation of downstream signaling pathways, including NF-κB. Therefore, TLR4 dimerization may be one of the first lines of regulation in activating LPS-induced signaling pathways. We report here biochemical evidence that the ethyl acetate fraction of garlic inhibited the LPS-induced dimerization of TLR4, resulting in the inhibition of NF-κB activation and the expression of cyclooxygenase 2 and inducible nitric oxide synthase. Our results demonstrate for the first time that a garlic extract can directly inhibit the TLRs-mediated signaling pathway at the receptor level. These results shed a new insight into understanding how garlic modulates the immune responses that could modify the risk of many chronic diseases.

Original languageEnglish (US)
Pages (from-to)368-375
Number of pages8
JournalBioscience, Biotechnology and Biochemistry
Volume72
Issue number2
DOIs
StatePublished - 2008

Fingerprint

Toll-Like Receptor 4
Garlic
dimerization
Dimerization
Allium sativum
garlic
lipopolysaccharides
Lipopolysaccharides
NF-kappa B
Toll-Like Receptors
Chemical activation
extracts
Nitric oxide
Nitric Oxide Synthase Type II
Cyclooxygenase 2
Sulfur
Medicine
Sulfur Compounds
receptors
Traditional Medicine

Keywords

  • Cyclooxygenase
  • Garlic
  • Nitric oxide synthase
  • Toll-like receptor

ASJC Scopus subject areas

  • Bioengineering
  • Biotechnology
  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry (miscellaneous)
  • Applied Microbiology and Biotechnology
  • Food Science

Cite this

Garlic (Allium sativum) extract inhibits lipopolysaccharide-induced toll-like receptor 4 dimerization. / Youn, Hyung Sun; Lim, Hyo Jin; Lee, Hwa Jin; Hwang, Daniel; Yang, Mihi; Jeon, Raok; Ryu, Jae Ha.

In: Bioscience, Biotechnology and Biochemistry, Vol. 72, No. 2, 2008, p. 368-375.

Research output: Contribution to journalArticle

Youn, Hyung Sun ; Lim, Hyo Jin ; Lee, Hwa Jin ; Hwang, Daniel ; Yang, Mihi ; Jeon, Raok ; Ryu, Jae Ha. / Garlic (Allium sativum) extract inhibits lipopolysaccharide-induced toll-like receptor 4 dimerization. In: Bioscience, Biotechnology and Biochemistry. 2008 ; Vol. 72, No. 2. pp. 368-375.
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