Gamete and Embryo Manipulation

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

This chapter summarizes contemporary techniques of manipulation of mouse gametes and embryos. In this context, "manipulation" refers to the creation by assisted reproduction, for example, in vitro fertilization, artificial insemination, and intracytoplasmic sperm injection; genetic modification, for example, transgenesis and gene targeting; preservation, for example, cryopreservation; rederivation, such as embryo transfer; and cloning, such as intracytoplasmic nuclear injection, of mouse embryos. Assisted reproduction refers to any procedure that ultimately results in the creation of a diploid embryo using a non-natural means to combine male (sperm) and female (oocytes) gametes. This chapter summarizes the techniques applied to derive viable embryos with the potential to develop into liveborn offspring. In each category, the purpose, applications, advantages, and disadvantages of each procedure are discussed in the chapter. The chapter further summarizes the techniques that are most often applied to manipulation of embryos to create genetically altered mouse strains.

Original languageEnglish (US)
Title of host publicationThe Mouse in Biomedical Research
PublisherElsevier Inc.
Pages211-224
Number of pages14
Volume1
ISBN (Print)9780123694546
DOIs
StatePublished - 2007

Fingerprint

Germ Cells
germ cells
assisted reproductive technologies
Embryonic Structures
mice
intracytoplasmic sperm injection
in vitro fertilization
gene targeting
Reproduction
embryo transfer
genetic engineering
artificial insemination
cryopreservation
molecular cloning
diploidy
oocytes
Gene Transfer Techniques
methodology
Artificial Insemination
spermatozoa

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Lloyd, K. C. K. (2007). Gamete and Embryo Manipulation. In The Mouse in Biomedical Research (Vol. 1, pp. 211-224). Elsevier Inc.. https://doi.org/10.1016/B978-012369454-6/50023-6

Gamete and Embryo Manipulation. / Lloyd, Kevin C K.

The Mouse in Biomedical Research. Vol. 1 Elsevier Inc., 2007. p. 211-224.

Research output: Chapter in Book/Report/Conference proceedingChapter

Lloyd, KCK 2007, Gamete and Embryo Manipulation. in The Mouse in Biomedical Research. vol. 1, Elsevier Inc., pp. 211-224. https://doi.org/10.1016/B978-012369454-6/50023-6
Lloyd KCK. Gamete and Embryo Manipulation. In The Mouse in Biomedical Research. Vol. 1. Elsevier Inc. 2007. p. 211-224 https://doi.org/10.1016/B978-012369454-6/50023-6
Lloyd, Kevin C K. / Gamete and Embryo Manipulation. The Mouse in Biomedical Research. Vol. 1 Elsevier Inc., 2007. pp. 211-224
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