Galectin-9 Is Critical for Mucosal Adaptive Immunity through the T Helper 17–IgA Axis

Chih Chia Liang, Chi Shan Li, I. Chun Weng, Huan Yuan Chen, Hsueh Han Lu, Chiu Ching Huang, Fu-Tong Liu

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Impairment of the intestinal mucosal immunity significantly increases the risk of acute and chronic diseases. IgA plays a major role in humoral mucosal immunity to provide protection against pathogens and toxins in the gut. Here, we investigated the role of endogenous galectin-9, a tandem repeat-type β-galactoside–binding protein, in intestinal mucosal immunity. By mucosal immunization of Lgals9−/− and littermate control mice, it was found that lack of galectin-9 impaired mucosal antigen-specific IgA response in the gut. Moreover, Lgals9−/− mice were more susceptible to developing watery diarrhea and more prone to death in response to high-dose cholera toxin. The results indicate the importance of galectin-9 in modulating intestinal adaptive immunity. Furthermore, bone marrow chimera mice were established, and galectin-9 in hematopoietic cells was found to be critical for adaptive IgA response. In addition, immunized Lgals9−/− mice exhibited lower expression of Il17 and fewer T helper 17 (Th17) cells in the lamina propria, implying that the Th17–IgA axis is involved in this mechanism. Taken together, these findings suggest that galectin-9 plays a role in mucosal adaptive immunity through the Th17–IgA axis. By manipulating the expression or activity of galectin-9, intestinal mucosal immune response can be altered and may benefit the development of mucosal vaccination.

Original languageEnglish (US)
Pages (from-to)1225-1235
Number of pages11
JournalAmerican Journal of Pathology
Volume188
Issue number5
DOIs
StatePublished - May 1 2018

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Galectins
Mucosal Immunity
Adaptive Immunity
Immunoglobulin A
Th17 Cells
Tandem Repeat Sequences
Cholera Toxin
Acute Disease
Humoral Immunity
Diarrhea
Immunization
Mucous Membrane
Vaccination
Chronic Disease
Bone Marrow
Antigens

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Liang, C. C., Li, C. S., Weng, I. C., Chen, H. Y., Lu, H. H., Huang, C. C., & Liu, F-T. (2018). Galectin-9 Is Critical for Mucosal Adaptive Immunity through the T Helper 17–IgA Axis. American Journal of Pathology, 188(5), 1225-1235. https://doi.org/10.1016/j.ajpath.2018.01.017

Galectin-9 Is Critical for Mucosal Adaptive Immunity through the T Helper 17–IgA Axis. / Liang, Chih Chia; Li, Chi Shan; Weng, I. Chun; Chen, Huan Yuan; Lu, Hsueh Han; Huang, Chiu Ching; Liu, Fu-Tong.

In: American Journal of Pathology, Vol. 188, No. 5, 01.05.2018, p. 1225-1235.

Research output: Contribution to journalArticle

Liang, CC, Li, CS, Weng, IC, Chen, HY, Lu, HH, Huang, CC & Liu, F-T 2018, 'Galectin-9 Is Critical for Mucosal Adaptive Immunity through the T Helper 17–IgA Axis', American Journal of Pathology, vol. 188, no. 5, pp. 1225-1235. https://doi.org/10.1016/j.ajpath.2018.01.017
Liang, Chih Chia ; Li, Chi Shan ; Weng, I. Chun ; Chen, Huan Yuan ; Lu, Hsueh Han ; Huang, Chiu Ching ; Liu, Fu-Tong. / Galectin-9 Is Critical for Mucosal Adaptive Immunity through the T Helper 17–IgA Axis. In: American Journal of Pathology. 2018 ; Vol. 188, No. 5. pp. 1225-1235.
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