Galectin-7 as a potential mediator of corneal epithelial cell migration

Zhiyi Cao, Neveen Said, Helen K. Wu, Ichiro Kuwabara, Fu-Tong Liu, Noorjahan Panjwani

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Objective: To assess the role of a carbohydrate-binding protein, galectin-7, in reepithelialization of corneal wounds. Methods: Transepithelial excimer laser ablations were performed on mouse corneas, and the wounds were allowed to partially heal in vivo for 18 to 22 hours. At the end of the healing period, expression levels of galectin-7 messenger RNA and protein were analyzed using semiquantitative reverse transcriptase-polymerase chain reaction, Western blot analysis, and immunohistochemical localization studies. To determine the effect of exogenous galectin-7 on reepithelialization of corneal wounds, corneas with 2-mm alkali burn wounds were allowed to partially heal in vitro for 20 to 24 hours in serum-free media in the presence or absence of recombinant galectin-7. At the end of the healing period, the wound areas were photographed and quantified. Results: Expression of galectin-7 messenger RNA and protein was markedly up-regulated in the corneal epithelium after injury. Exogenous galectin-7 stimulated reepithelialization of corneal wounds. The stimulatory effect of galectin-7 on corneal epithelial wound closure was specifically inhibited by a competing sugar, β-lactose, but not by an irrelevant disaccharide, sucrose. Conclusions: Galectin-7 has the potential to mediate corneal epithelial cell migration and reepithelialization of wounds. Clinical Relevance: These findings have broad implications for developing novel, galectin-based, therapeutic strategies for treatment of nonhealing corneal epithelial defects.

Original languageEnglish (US)
Pages (from-to)82-86
Number of pages5
JournalArchives of Ophthalmology
Volume121
Issue number1
StatePublished - Jan 1 2003

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Galectins
Cell Movement
Epithelial Cells
Wounds and Injuries
Cornea
Excimer Lasers
Messenger RNA
Corneal Epithelium
Disaccharides
Serum-Free Culture Media
Alkalies
Laser Therapy
Lactose
Reverse Transcriptase Polymerase Chain Reaction
Wound Healing
Sucrose
Proteins
Western Blotting

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Cao, Z., Said, N., Wu, H. K., Kuwabara, I., Liu, F-T., & Panjwani, N. (2003). Galectin-7 as a potential mediator of corneal epithelial cell migration. Archives of Ophthalmology, 121(1), 82-86.

Galectin-7 as a potential mediator of corneal epithelial cell migration. / Cao, Zhiyi; Said, Neveen; Wu, Helen K.; Kuwabara, Ichiro; Liu, Fu-Tong; Panjwani, Noorjahan.

In: Archives of Ophthalmology, Vol. 121, No. 1, 01.01.2003, p. 82-86.

Research output: Contribution to journalArticle

Cao, Z, Said, N, Wu, HK, Kuwabara, I, Liu, F-T & Panjwani, N 2003, 'Galectin-7 as a potential mediator of corneal epithelial cell migration', Archives of Ophthalmology, vol. 121, no. 1, pp. 82-86.
Cao Z, Said N, Wu HK, Kuwabara I, Liu F-T, Panjwani N. Galectin-7 as a potential mediator of corneal epithelial cell migration. Archives of Ophthalmology. 2003 Jan 1;121(1):82-86.
Cao, Zhiyi ; Said, Neveen ; Wu, Helen K. ; Kuwabara, Ichiro ; Liu, Fu-Tong ; Panjwani, Noorjahan. / Galectin-7 as a potential mediator of corneal epithelial cell migration. In: Archives of Ophthalmology. 2003 ; Vol. 121, No. 1. pp. 82-86.
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