Galectin-3 stimulates preadipocyte proliferation and is up-regulated in growing adipose tissue

Kohji Kiwaki, Colleen M. Novak, Daniel K. Hsu, Fu-Tong Liu, James A. Levine

Research output: Contribution to journalArticle

45 Scopus citations

Abstract

Objective: Some cytokines and mediators of inflammation can alter adiposity through their effects on adipocyte number. To probe the molecular basis of obesity, this study determined whether galectin-3 was present in adipose tissue and investigated its effects on fat cell number. Research Methods and Procedures: In the first study, obesity-prone C57BL/6J mice were fed with high-fat (58%) diet. Epididymal fat pads were collected at Day 0, Day 60, and Day 120 after the start of high-fat feeding. Results: Levels of adipocyte galectin-3 protein, determined using Western blot analysis, increased as the mice became obese. Galectin-3 mRNA and protein were then detected in human adipose tissue, primarily in the preadipocyte fraction. It was found that recombinant human galectin-3 stimulated proliferation of primary cultured preadipocytes as well as DNA synthesis through lectin-carbohydrate interaction. Discussion: Galectin-3, which has been known to play a versatile role especially in immune cells, might play a role also in adipose tissue and be associated with the pathophysiology of obesity.

Original languageEnglish (US)
Pages (from-to)32-39
Number of pages8
JournalObesity
Volume15
Issue number1
DOIs
StatePublished - Jan 2007

Keywords

  • C57BL/6J mice
  • Diet
  • Obesity phenotypes

ASJC Scopus subject areas

  • Endocrinology
  • Medicine (miscellaneous)
  • Endocrinology, Diabetes and Metabolism
  • Nutrition and Dietetics

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