Galectin-3 plays an important role in innate immunity to gastric infection by Helicobacter pylori

Ah Mee Park, Satoru Hagiwara, Daniel K. Hsu, Fu-Tong Liu, Osamu Yoshie

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

We studied the role of galectin-3 (Gal3) in gastric infection by Helicobacter pylori. We first demonstrated that Gal3 was selectively expressed by gastric surface epithelial cells and abundantly secreted into the surface mucus layer. We next inoculated H. pylori Sydney strain 1 into wild-type (WT) and Gal3-deficient mice using a stomach tube. At 2 weeks postinoculation, the bacterial cells were mostly trapped within the surface mucus layer in WT mice. In sharp contrast, they infiltrated deep into the gastric glands in Gal3-deficient mice. Bacterial loads in the gastric tissues were also much higher in Gal3-deficient mice than in WT mice. At 6 months postinoculation, H. pylori had successfully colonized within the gastric glands of both WT and Gal3-deficient mice, although the bacterial loads were still higher in the latter. Furthermore, large lymphoid clusters mostly consisting of B cells were frequently observed in the gastric submucosa of Gal3-deficient mice. In vitro, peritoneal macrophages from Gal3-deficient mice were inefficient in killing engulfed H. pylori. Furthermore, recombinant Gal3 not only induced rapid aggregation of H. pylori but also exerted a potent bactericidal effect on H. pylori as revealed by propidium iodide uptake and a morphological shift from spiral to coccoid form. However, a minor fraction of bacterial cells, probably transient phase variants of Gal3-binding sugar moieties, escaped killing by Gal3. Collectively, our data demonstrate that Gal3 plays an important role in innate immunity to infection and colonization of H. pylori.

Original languageEnglish (US)
Pages (from-to)1184-1193
Number of pages10
JournalInfection and Immunity
Volume84
Issue number4
DOIs
StatePublished - Apr 1 2016

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Galectin 3
Innate Immunity
Helicobacter pylori
Stomach
Infection
Bacterial Load
Mucus
Gastric Mucosa
Propidium
Peritoneal Macrophages

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

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Galectin-3 plays an important role in innate immunity to gastric infection by Helicobacter pylori. / Park, Ah Mee; Hagiwara, Satoru; Hsu, Daniel K.; Liu, Fu-Tong; Yoshie, Osamu.

In: Infection and Immunity, Vol. 84, No. 4, 01.04.2016, p. 1184-1193.

Research output: Contribution to journalArticle

Park, Ah Mee ; Hagiwara, Satoru ; Hsu, Daniel K. ; Liu, Fu-Tong ; Yoshie, Osamu. / Galectin-3 plays an important role in innate immunity to gastric infection by Helicobacter pylori. In: Infection and Immunity. 2016 ; Vol. 84, No. 4. pp. 1184-1193.
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