Galectin-3 plays a modulatory role in the life span and activation of murine neutrophils during early Toxoplasma gondii infection

Celene M O S Alves, Deise A O Silva, Ana Elisa C S Azzolini, Cleni M. Marzocchi-Machado, Julianne V. Carvalho, Ana Cláudia A M Pajuaba, Yara Maria Lucisano-Valim, Roger Chammas, Fu-Tong Liu, Maria C. Roque-Barreira, José R. Mineo

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Abstract

Galectins are Β-galactoside-binding lectins involved in several biological processes and galectin-3 (Gal-3) is related to modulation of immune and inflammatory responses. This study aimed to evaluate the role of Gal-3 in the life span and biological functions of murine neutrophils during in vitro infection by virulent Toxoplasma gondii RH strain. Inflammatory peritoneal neutrophils (N) from C57BL/6 wild-type (WT) and Gal-3 knockout (KO) mice were cultured in the presence or absence of parasites and analyzed for phosphatidylserine (PS) exposure and cell death using Annexin-V and propidium iodide staining, and cell viability by MTT assay. Cell toxicities determined by lactate dehydrogenase (LDH), degranulation by lysozyme release, and cytokine production were measured in N culture supernatants. Phorbol myristate acetate (PMA)- or zymosan-dependent reactive oxygen species (ROS) were measured in N cultures. Our results demonstrated that Gal-3 is involved in the increase of the viable N number and the decrease of PS exposure and cell death following T. gondii infection. We also observed that Gal-3 downmodulates T. gondii-induced N toxicity as well as N degranulation regardless of infection. Furthermore, Gal-3 expression by N was associated with increased levels of IL-10 in the beginning and decreased levels of TNF-α later on, regardless of parasite infection, as well as with decreased levels of IL-6 and increased IL-12 levels, following early parasite infection. Our results also showed that Gal-3 suppresses PMA- but not zymosan-induced ROS generation in N following T. gondii infection. In conclusion, Gal-3 plays an important modulatory role by interfering in N life span and activation during early T. gondii infection.

Original languageEnglish (US)
Pages (from-to)475-485
Number of pages11
JournalImmunobiology
Volume215
Issue number6
DOIs
StatePublished - Jun 2010

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Keywords

  • Apoptosis
  • Cell viability
  • Galectin-3
  • Neutrophils
  • Reactive oxygen species
  • Toxoplasma gondii

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Hematology

Cite this

Alves, C. M. O. S., Silva, D. A. O., Azzolini, A. E. C. S., Marzocchi-Machado, C. M., Carvalho, J. V., Pajuaba, A. C. A. M., Lucisano-Valim, Y. M., Chammas, R., Liu, F-T., Roque-Barreira, M. C., & Mineo, J. R. (2010). Galectin-3 plays a modulatory role in the life span and activation of murine neutrophils during early Toxoplasma gondii infection. Immunobiology, 215(6), 475-485. https://doi.org/10.1016/j.imbio.2009.08.001