Abstract
Heart failure with preserved ejection fraction (HFPEF) is characterized by myocardial interstitial fibrosis. A total of 146 patients with HFPEF, were recruited. HFPEF severity was determined using Doppler imaging (E/Em) and also cardiac magnetic resonance imaging (CMRI). Canine modeling of HFPEF was induced by aortic banding. Hemodynamic and echocardiographic data were obtained before and after pressure loading and myocardial Galectin-3 was determined. Mechanical stretch of cultured cardiomyocytes served as the cellular model of HFPEF. Patients with severe HFPEF had significantly higher plasma Galectin-3 levels. Significant correlation between plasma Galectin-3 and E/Em in advanced HFPEF patients was noted. After 2 weeks of pressure overload in canine models, the protein expression of Galectin-3 from LV myocardial tissue was significantly increased (p <0.01) compared with controls. Galectin-3 expression paralleled the severity of LV diastolic dysfunction by evaluation of CMRI (r =-0.58, p = 0.003) and tissue fibrosis (r = 0.59, p = 0.002). After adjusting for confounders for diastolic dysfunction, Galectin-3 levels were still associated with diastolic parameters both in humans (p <0.001) and canine model (p = 0.041). Mechanical stretch increased Galectin-3 secretion in cultured cardiomyocytes. Both plasma and myocardial Galectin-3 levels correlated with severity of cardiac diastolic dysfunction.
| Original language | English (US) |
|---|---|
| Article number | 17007 |
| Journal | Scientific Reports |
| Volume | 5 |
| DOIs | |
| State | Published - Nov 19 2015 |
| Externally published | Yes |
Fingerprint
ASJC Scopus subject areas
- General
Cite this
Galectin-3 level and the severity of cardiac diastolic dysfunction using cellular and animal models and clinical indices. / Wu, Cho Kai; Su, Mao Yuan; Lee, Jen Kuang; Chiang, Fu Tien; Hwang, Juey Jen; Lin, Jiunn Lee; Chen, Jin Jer; Liu, Fu-Tong; Tsai, Chia Ti.
In: Scientific Reports, Vol. 5, 17007, 19.11.2015.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Galectin-3 level and the severity of cardiac diastolic dysfunction using cellular and animal models and clinical indices
AU - Wu, Cho Kai
AU - Su, Mao Yuan
AU - Lee, Jen Kuang
AU - Chiang, Fu Tien
AU - Hwang, Juey Jen
AU - Lin, Jiunn Lee
AU - Chen, Jin Jer
AU - Liu, Fu-Tong
AU - Tsai, Chia Ti
PY - 2015/11/19
Y1 - 2015/11/19
N2 - Heart failure with preserved ejection fraction (HFPEF) is characterized by myocardial interstitial fibrosis. A total of 146 patients with HFPEF, were recruited. HFPEF severity was determined using Doppler imaging (E/Em) and also cardiac magnetic resonance imaging (CMRI). Canine modeling of HFPEF was induced by aortic banding. Hemodynamic and echocardiographic data were obtained before and after pressure loading and myocardial Galectin-3 was determined. Mechanical stretch of cultured cardiomyocytes served as the cellular model of HFPEF. Patients with severe HFPEF had significantly higher plasma Galectin-3 levels. Significant correlation between plasma Galectin-3 and E/Em in advanced HFPEF patients was noted. After 2 weeks of pressure overload in canine models, the protein expression of Galectin-3 from LV myocardial tissue was significantly increased (p <0.01) compared with controls. Galectin-3 expression paralleled the severity of LV diastolic dysfunction by evaluation of CMRI (r =-0.58, p = 0.003) and tissue fibrosis (r = 0.59, p = 0.002). After adjusting for confounders for diastolic dysfunction, Galectin-3 levels were still associated with diastolic parameters both in humans (p <0.001) and canine model (p = 0.041). Mechanical stretch increased Galectin-3 secretion in cultured cardiomyocytes. Both plasma and myocardial Galectin-3 levels correlated with severity of cardiac diastolic dysfunction.
AB - Heart failure with preserved ejection fraction (HFPEF) is characterized by myocardial interstitial fibrosis. A total of 146 patients with HFPEF, were recruited. HFPEF severity was determined using Doppler imaging (E/Em) and also cardiac magnetic resonance imaging (CMRI). Canine modeling of HFPEF was induced by aortic banding. Hemodynamic and echocardiographic data were obtained before and after pressure loading and myocardial Galectin-3 was determined. Mechanical stretch of cultured cardiomyocytes served as the cellular model of HFPEF. Patients with severe HFPEF had significantly higher plasma Galectin-3 levels. Significant correlation between plasma Galectin-3 and E/Em in advanced HFPEF patients was noted. After 2 weeks of pressure overload in canine models, the protein expression of Galectin-3 from LV myocardial tissue was significantly increased (p <0.01) compared with controls. Galectin-3 expression paralleled the severity of LV diastolic dysfunction by evaluation of CMRI (r =-0.58, p = 0.003) and tissue fibrosis (r = 0.59, p = 0.002). After adjusting for confounders for diastolic dysfunction, Galectin-3 levels were still associated with diastolic parameters both in humans (p <0.001) and canine model (p = 0.041). Mechanical stretch increased Galectin-3 secretion in cultured cardiomyocytes. Both plasma and myocardial Galectin-3 levels correlated with severity of cardiac diastolic dysfunction.
UR - http://www.scopus.com/inward/record.url?scp=84947583689&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84947583689&partnerID=8YFLogxK
U2 - 10.1038/srep17007
DO - 10.1038/srep17007
M3 - Article
VL - 5
JO - Scientific Reports
JF - Scientific Reports
SN - 2045-2322
M1 - 17007
ER -