Galectin-3 is strongly up-regulated in nonapoptosing mammary epithelial cells during rat mammary gland involution

Jörg Mengwasser, Fu-Tong Liu, Jonathan P. Sleeman

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Galectin-3 is an endogenous mammalian lectin that binds to ABH carbohydrate antigens. Here we show that galectin-3 is strongly up-regulated during mammary gland involution and that it is expressed virtually exclusively on nonapoptotic cells. We demonstrate that dexamethasone, an inhibitor of the second phase of mammary gland involution, potently suppresses up-regulation of galectin-3 as judged immunohistochemically and on western blots, suggesting that systemic hormone levels regulate galectin-3 expression during involution. However, at the RNA level galectin-3 expression is rapidly up-regulated on the onset of involution but remains consistantly high during the first and second phase of involution regardless of dexamethasone treatment. These data suggest that the up-regulation of galectin-3 in the involuting mammary gland is not only controlled transcriptionally but also regulated posttranscriptionally under the control of systemic glucocorticoid hormones involved in coordinating the involution process.

Original languageEnglish (US)
Pages (from-to)129-134
Number of pages6
JournalGlycobiology
Volume12
Issue number2
StatePublished - 2002

Fingerprint

Galectin 3
Human Mammary Glands
Rats
Breast
Epithelial Cells
Dexamethasone
Up-Regulation
Hormones
Lectins
Glucocorticoids
Western Blotting
Carbohydrates
RNA
Antigens

Keywords

  • ABH histo-blood group antigen
  • Apoptosis
  • Galectin-3
  • Mammary gland
  • Tissue remodeling

ASJC Scopus subject areas

  • Biochemistry

Cite this

Galectin-3 is strongly up-regulated in nonapoptosing mammary epithelial cells during rat mammary gland involution. / Mengwasser, Jörg; Liu, Fu-Tong; Sleeman, Jonathan P.

In: Glycobiology, Vol. 12, No. 2, 2002, p. 129-134.

Research output: Contribution to journalArticle

Mengwasser, Jörg ; Liu, Fu-Tong ; Sleeman, Jonathan P. / Galectin-3 is strongly up-regulated in nonapoptosing mammary epithelial cells during rat mammary gland involution. In: Glycobiology. 2002 ; Vol. 12, No. 2. pp. 129-134.
@article{6dbacbfb456b4c61b7c3329322eabfd7,
title = "Galectin-3 is strongly up-regulated in nonapoptosing mammary epithelial cells during rat mammary gland involution",
abstract = "Galectin-3 is an endogenous mammalian lectin that binds to ABH carbohydrate antigens. Here we show that galectin-3 is strongly up-regulated during mammary gland involution and that it is expressed virtually exclusively on nonapoptotic cells. We demonstrate that dexamethasone, an inhibitor of the second phase of mammary gland involution, potently suppresses up-regulation of galectin-3 as judged immunohistochemically and on western blots, suggesting that systemic hormone levels regulate galectin-3 expression during involution. However, at the RNA level galectin-3 expression is rapidly up-regulated on the onset of involution but remains consistantly high during the first and second phase of involution regardless of dexamethasone treatment. These data suggest that the up-regulation of galectin-3 in the involuting mammary gland is not only controlled transcriptionally but also regulated posttranscriptionally under the control of systemic glucocorticoid hormones involved in coordinating the involution process.",
keywords = "ABH histo-blood group antigen, Apoptosis, Galectin-3, Mammary gland, Tissue remodeling",
author = "J{\"o}rg Mengwasser and Fu-Tong Liu and Sleeman, {Jonathan P.}",
year = "2002",
language = "English (US)",
volume = "12",
pages = "129--134",
journal = "Glycobiology",
issn = "0959-6658",
publisher = "Oxford University Press",
number = "2",

}

TY - JOUR

T1 - Galectin-3 is strongly up-regulated in nonapoptosing mammary epithelial cells during rat mammary gland involution

AU - Mengwasser, Jörg

AU - Liu, Fu-Tong

AU - Sleeman, Jonathan P.

PY - 2002

Y1 - 2002

N2 - Galectin-3 is an endogenous mammalian lectin that binds to ABH carbohydrate antigens. Here we show that galectin-3 is strongly up-regulated during mammary gland involution and that it is expressed virtually exclusively on nonapoptotic cells. We demonstrate that dexamethasone, an inhibitor of the second phase of mammary gland involution, potently suppresses up-regulation of galectin-3 as judged immunohistochemically and on western blots, suggesting that systemic hormone levels regulate galectin-3 expression during involution. However, at the RNA level galectin-3 expression is rapidly up-regulated on the onset of involution but remains consistantly high during the first and second phase of involution regardless of dexamethasone treatment. These data suggest that the up-regulation of galectin-3 in the involuting mammary gland is not only controlled transcriptionally but also regulated posttranscriptionally under the control of systemic glucocorticoid hormones involved in coordinating the involution process.

AB - Galectin-3 is an endogenous mammalian lectin that binds to ABH carbohydrate antigens. Here we show that galectin-3 is strongly up-regulated during mammary gland involution and that it is expressed virtually exclusively on nonapoptotic cells. We demonstrate that dexamethasone, an inhibitor of the second phase of mammary gland involution, potently suppresses up-regulation of galectin-3 as judged immunohistochemically and on western blots, suggesting that systemic hormone levels regulate galectin-3 expression during involution. However, at the RNA level galectin-3 expression is rapidly up-regulated on the onset of involution but remains consistantly high during the first and second phase of involution regardless of dexamethasone treatment. These data suggest that the up-regulation of galectin-3 in the involuting mammary gland is not only controlled transcriptionally but also regulated posttranscriptionally under the control of systemic glucocorticoid hormones involved in coordinating the involution process.

KW - ABH histo-blood group antigen

KW - Apoptosis

KW - Galectin-3

KW - Mammary gland

KW - Tissue remodeling

UR - http://www.scopus.com/inward/record.url?scp=0036237255&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036237255&partnerID=8YFLogxK

M3 - Article

C2 - 11886846

AN - SCOPUS:0036237255

VL - 12

SP - 129

EP - 134

JO - Glycobiology

JF - Glycobiology

SN - 0959-6658

IS - 2

ER -