Galectin-3 functions as an adhesion molecule to support eosinophil rolling and adhesion under conditions of flow

Savita P. Rao, Zhuangzhi Wang, Riaz I. Zuberi, Lyudmila Sikora, Nooshin S. Bahaie, Bruce L. Zuraw, Fu-Tong Liu, P. Sriramarao

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Abstract

Allergic inflammation involves the mobilization and trafficking of eosinophils to sites of inflammation. Galectin-3 (Gal-3) has been shown to play a critical role in eosinophil recruitment and airway allergic inflammation in vivo. The role played by Gal-3 in human eosinophil trafficking was investigated. Eosinophils from allergic donors expressed elevated levels of Gal-3 and demonstrated significantly increased rolling and firm adhesion on immobilized VCAM-1 and, more surprisingly, on Gal-3 under conditions of flow. Inhibition studies with specific mAbs as well as lactose demonstrated that: 1) eosinophil-expressed Gal-3 mediates rolling and adhesion on VCAM-1; 2) α4 integrin mediates eosinophil rolling on immobilized Gal-3; and 3) eosinophil-expressed Gal-3 interacts with immobilized Gal-3 through the carbohydrate recognition domain of Gal-3 during eosinophil trafficking. These findings were further confirmed using inflamed endothelial cells. Interestingly, Gal-3 was found to bind to α4 integrin by ELISA, and the two molecules exhibited colocalized expression on the cell surface of eosinophils from allergic donors. These findings suggest that Gal-3 functions as a cell surface adhesion molecule to support eosinophil rolling and adhesion under conditions of flow.

Original languageEnglish (US)
Pages (from-to)7800-7807
Number of pages8
JournalJournal of Immunology
Volume179
Issue number11
StatePublished - Dec 1 2007

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ASJC Scopus subject areas

  • Immunology

Cite this

Rao, S. P., Wang, Z., Zuberi, R. I., Sikora, L., Bahaie, N. S., Zuraw, B. L., Liu, F-T., & Sriramarao, P. (2007). Galectin-3 functions as an adhesion molecule to support eosinophil rolling and adhesion under conditions of flow. Journal of Immunology, 179(11), 7800-7807.