Galectin-3 down-regulates IL-5 gene expression on different cell types

Isabel Cortegano, Victoria Del Pozo, Blanca Cárdaba, Belén De Andrés, Soledad Gallardo, Ana Del Amo, Ignacio Arrieta, Aurora Jurado, Pilar Palomino, Fu-Tong Liu, Carlos Lahoz

Research output: Contribution to journalArticle

94 Citations (Scopus)

Abstract

Galectin-3 is an animal lectin, formerly named ε-binding protein or Mac-2, which has been described to play an important role in some inflammatory processes by the implication of different cells and the increase in cell adhesion functions through laminin binding activity. In this work we analyzed the role of galectin-3 in the modulation of Th2 cytokines that have an important role in the development of the inflammatory response. We have found that the addition of galectin-3 to human eosinophils, the eosinophilic cell line EoL-3, PBMC, and an Ag-specific T cell line (CD4+) produced a selective inhibition of IL-5 transcription. No inhibitory effect was found on the IL-4 mRNA transcription rate. The inhibitory effect on IL-5 transcription was reversed by incubation with lactose and using specific Ah against galectin-3. Galectin-3 is able to induce inhibition of the IL-5 released in the supernatants from PBMC stimulated with phorbol 12,13-dibutyrate and anti- CD3. Similar results were obtained when a T-specific cell line was stimulated with Ag. Also, EoL-3 stimulated with anti-CD32 produced IL-5 protein, the synthesis of which was partially inhibited by galectin-3. The present results demonstrate that galectin-3 induces a selective down-regulation of IL-5 expression in different cell types, opening important new possibilities in the regulation of the allergic reactions.

Original languageEnglish (US)
Pages (from-to)385-389
Number of pages5
JournalJournal of Immunology
Volume161
Issue number1
StatePublished - 1998

Fingerprint

Galectin 3
Interleukin-5
Down-Regulation
Gene Expression
Cell Line
Phorbol 12,13-Dibutyrate
Laminin
Lactose
Eosinophils
Lectins
Cell Adhesion
Interleukin-4
Carrier Proteins
Hypersensitivity
Cytokines
T-Lymphocytes
Messenger RNA
Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Cortegano, I., Del Pozo, V., Cárdaba, B., De Andrés, B., Gallardo, S., Del Amo, A., ... Lahoz, C. (1998). Galectin-3 down-regulates IL-5 gene expression on different cell types. Journal of Immunology, 161(1), 385-389.

Galectin-3 down-regulates IL-5 gene expression on different cell types. / Cortegano, Isabel; Del Pozo, Victoria; Cárdaba, Blanca; De Andrés, Belén; Gallardo, Soledad; Del Amo, Ana; Arrieta, Ignacio; Jurado, Aurora; Palomino, Pilar; Liu, Fu-Tong; Lahoz, Carlos.

In: Journal of Immunology, Vol. 161, No. 1, 1998, p. 385-389.

Research output: Contribution to journalArticle

Cortegano, I, Del Pozo, V, Cárdaba, B, De Andrés, B, Gallardo, S, Del Amo, A, Arrieta, I, Jurado, A, Palomino, P, Liu, F-T & Lahoz, C 1998, 'Galectin-3 down-regulates IL-5 gene expression on different cell types', Journal of Immunology, vol. 161, no. 1, pp. 385-389.
Cortegano I, Del Pozo V, Cárdaba B, De Andrés B, Gallardo S, Del Amo A et al. Galectin-3 down-regulates IL-5 gene expression on different cell types. Journal of Immunology. 1998;161(1):385-389.
Cortegano, Isabel ; Del Pozo, Victoria ; Cárdaba, Blanca ; De Andrés, Belén ; Gallardo, Soledad ; Del Amo, Ana ; Arrieta, Ignacio ; Jurado, Aurora ; Palomino, Pilar ; Liu, Fu-Tong ; Lahoz, Carlos. / Galectin-3 down-regulates IL-5 gene expression on different cell types. In: Journal of Immunology. 1998 ; Vol. 161, No. 1. pp. 385-389.
@article{dc9bd7852bbb415d927cf8d08a4632de,
title = "Galectin-3 down-regulates IL-5 gene expression on different cell types",
abstract = "Galectin-3 is an animal lectin, formerly named ε-binding protein or Mac-2, which has been described to play an important role in some inflammatory processes by the implication of different cells and the increase in cell adhesion functions through laminin binding activity. In this work we analyzed the role of galectin-3 in the modulation of Th2 cytokines that have an important role in the development of the inflammatory response. We have found that the addition of galectin-3 to human eosinophils, the eosinophilic cell line EoL-3, PBMC, and an Ag-specific T cell line (CD4+) produced a selective inhibition of IL-5 transcription. No inhibitory effect was found on the IL-4 mRNA transcription rate. The inhibitory effect on IL-5 transcription was reversed by incubation with lactose and using specific Ah against galectin-3. Galectin-3 is able to induce inhibition of the IL-5 released in the supernatants from PBMC stimulated with phorbol 12,13-dibutyrate and anti- CD3. Similar results were obtained when a T-specific cell line was stimulated with Ag. Also, EoL-3 stimulated with anti-CD32 produced IL-5 protein, the synthesis of which was partially inhibited by galectin-3. The present results demonstrate that galectin-3 induces a selective down-regulation of IL-5 expression in different cell types, opening important new possibilities in the regulation of the allergic reactions.",
author = "Isabel Cortegano and {Del Pozo}, Victoria and Blanca C{\'a}rdaba and {De Andr{\'e}s}, Bel{\'e}n and Soledad Gallardo and {Del Amo}, Ana and Ignacio Arrieta and Aurora Jurado and Pilar Palomino and Fu-Tong Liu and Carlos Lahoz",
year = "1998",
language = "English (US)",
volume = "161",
pages = "385--389",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "1",

}

TY - JOUR

T1 - Galectin-3 down-regulates IL-5 gene expression on different cell types

AU - Cortegano, Isabel

AU - Del Pozo, Victoria

AU - Cárdaba, Blanca

AU - De Andrés, Belén

AU - Gallardo, Soledad

AU - Del Amo, Ana

AU - Arrieta, Ignacio

AU - Jurado, Aurora

AU - Palomino, Pilar

AU - Liu, Fu-Tong

AU - Lahoz, Carlos

PY - 1998

Y1 - 1998

N2 - Galectin-3 is an animal lectin, formerly named ε-binding protein or Mac-2, which has been described to play an important role in some inflammatory processes by the implication of different cells and the increase in cell adhesion functions through laminin binding activity. In this work we analyzed the role of galectin-3 in the modulation of Th2 cytokines that have an important role in the development of the inflammatory response. We have found that the addition of galectin-3 to human eosinophils, the eosinophilic cell line EoL-3, PBMC, and an Ag-specific T cell line (CD4+) produced a selective inhibition of IL-5 transcription. No inhibitory effect was found on the IL-4 mRNA transcription rate. The inhibitory effect on IL-5 transcription was reversed by incubation with lactose and using specific Ah against galectin-3. Galectin-3 is able to induce inhibition of the IL-5 released in the supernatants from PBMC stimulated with phorbol 12,13-dibutyrate and anti- CD3. Similar results were obtained when a T-specific cell line was stimulated with Ag. Also, EoL-3 stimulated with anti-CD32 produced IL-5 protein, the synthesis of which was partially inhibited by galectin-3. The present results demonstrate that galectin-3 induces a selective down-regulation of IL-5 expression in different cell types, opening important new possibilities in the regulation of the allergic reactions.

AB - Galectin-3 is an animal lectin, formerly named ε-binding protein or Mac-2, which has been described to play an important role in some inflammatory processes by the implication of different cells and the increase in cell adhesion functions through laminin binding activity. In this work we analyzed the role of galectin-3 in the modulation of Th2 cytokines that have an important role in the development of the inflammatory response. We have found that the addition of galectin-3 to human eosinophils, the eosinophilic cell line EoL-3, PBMC, and an Ag-specific T cell line (CD4+) produced a selective inhibition of IL-5 transcription. No inhibitory effect was found on the IL-4 mRNA transcription rate. The inhibitory effect on IL-5 transcription was reversed by incubation with lactose and using specific Ah against galectin-3. Galectin-3 is able to induce inhibition of the IL-5 released in the supernatants from PBMC stimulated with phorbol 12,13-dibutyrate and anti- CD3. Similar results were obtained when a T-specific cell line was stimulated with Ag. Also, EoL-3 stimulated with anti-CD32 produced IL-5 protein, the synthesis of which was partially inhibited by galectin-3. The present results demonstrate that galectin-3 induces a selective down-regulation of IL-5 expression in different cell types, opening important new possibilities in the regulation of the allergic reactions.

UR - http://www.scopus.com/inward/record.url?scp=7144226602&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=7144226602&partnerID=8YFLogxK

M3 - Article

C2 - 9647247

AN - SCOPUS:7144226602

VL - 161

SP - 385

EP - 389

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 1

ER -