Abstract
Galectin-3 is an animal lectin, formerly named ε-binding protein or Mac-2, which has been described to play an important role in some inflammatory processes by the implication of different cells and the increase in cell adhesion functions through laminin binding activity. In this work we analyzed the role of galectin-3 in the modulation of Th2 cytokines that have an important role in the development of the inflammatory response. We have found that the addition of galectin-3 to human eosinophils, the eosinophilic cell line EoL-3, PBMC, and an Ag-specific T cell line (CD4+) produced a selective inhibition of IL-5 transcription. No inhibitory effect was found on the IL-4 mRNA transcription rate. The inhibitory effect on IL-5 transcription was reversed by incubation with lactose and using specific Ah against galectin-3. Galectin-3 is able to induce inhibition of the IL-5 released in the supernatants from PBMC stimulated with phorbol 12,13-dibutyrate and anti- CD3. Similar results were obtained when a T-specific cell line was stimulated with Ag. Also, EoL-3 stimulated with anti-CD32 produced IL-5 protein, the synthesis of which was partially inhibited by galectin-3. The present results demonstrate that galectin-3 induces a selective down-regulation of IL-5 expression in different cell types, opening important new possibilities in the regulation of the allergic reactions.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 385-389 |
| Number of pages | 5 |
| Journal | Journal of Immunology |
| Volume | 161 |
| Issue number | 1 |
| State | Published - 1998 |
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ASJC Scopus subject areas
- Immunology
Cite this
Galectin-3 down-regulates IL-5 gene expression on different cell types. / Cortegano, Isabel; Del Pozo, Victoria; Cárdaba, Blanca; De Andrés, Belén; Gallardo, Soledad; Del Amo, Ana; Arrieta, Ignacio; Jurado, Aurora; Palomino, Pilar; Liu, Fu-Tong; Lahoz, Carlos.
In: Journal of Immunology, Vol. 161, No. 1, 1998, p. 385-389.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Galectin-3 down-regulates IL-5 gene expression on different cell types
AU - Cortegano, Isabel
AU - Del Pozo, Victoria
AU - Cárdaba, Blanca
AU - De Andrés, Belén
AU - Gallardo, Soledad
AU - Del Amo, Ana
AU - Arrieta, Ignacio
AU - Jurado, Aurora
AU - Palomino, Pilar
AU - Liu, Fu-Tong
AU - Lahoz, Carlos
PY - 1998
Y1 - 1998
N2 - Galectin-3 is an animal lectin, formerly named ε-binding protein or Mac-2, which has been described to play an important role in some inflammatory processes by the implication of different cells and the increase in cell adhesion functions through laminin binding activity. In this work we analyzed the role of galectin-3 in the modulation of Th2 cytokines that have an important role in the development of the inflammatory response. We have found that the addition of galectin-3 to human eosinophils, the eosinophilic cell line EoL-3, PBMC, and an Ag-specific T cell line (CD4+) produced a selective inhibition of IL-5 transcription. No inhibitory effect was found on the IL-4 mRNA transcription rate. The inhibitory effect on IL-5 transcription was reversed by incubation with lactose and using specific Ah against galectin-3. Galectin-3 is able to induce inhibition of the IL-5 released in the supernatants from PBMC stimulated with phorbol 12,13-dibutyrate and anti- CD3. Similar results were obtained when a T-specific cell line was stimulated with Ag. Also, EoL-3 stimulated with anti-CD32 produced IL-5 protein, the synthesis of which was partially inhibited by galectin-3. The present results demonstrate that galectin-3 induces a selective down-regulation of IL-5 expression in different cell types, opening important new possibilities in the regulation of the allergic reactions.
AB - Galectin-3 is an animal lectin, formerly named ε-binding protein or Mac-2, which has been described to play an important role in some inflammatory processes by the implication of different cells and the increase in cell adhesion functions through laminin binding activity. In this work we analyzed the role of galectin-3 in the modulation of Th2 cytokines that have an important role in the development of the inflammatory response. We have found that the addition of galectin-3 to human eosinophils, the eosinophilic cell line EoL-3, PBMC, and an Ag-specific T cell line (CD4+) produced a selective inhibition of IL-5 transcription. No inhibitory effect was found on the IL-4 mRNA transcription rate. The inhibitory effect on IL-5 transcription was reversed by incubation with lactose and using specific Ah against galectin-3. Galectin-3 is able to induce inhibition of the IL-5 released in the supernatants from PBMC stimulated with phorbol 12,13-dibutyrate and anti- CD3. Similar results were obtained when a T-specific cell line was stimulated with Ag. Also, EoL-3 stimulated with anti-CD32 produced IL-5 protein, the synthesis of which was partially inhibited by galectin-3. The present results demonstrate that galectin-3 induces a selective down-regulation of IL-5 expression in different cell types, opening important new possibilities in the regulation of the allergic reactions.
UR - http://www.scopus.com/inward/record.url?scp=7144226602&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=7144226602&partnerID=8YFLogxK
M3 - Article
C2 - 9647247
AN - SCOPUS:7144226602
VL - 161
SP - 385
EP - 389
JO - Journal of Immunology
JF - Journal of Immunology
SN - 0022-1767
IS - 1
ER -