Galectin-3 and laminin expression in neoplastic and non-neoplastic thyroid tissue

Pedro L. Fernández, María J. Merino, Mercedes Gómez, Elías Campo, Teresa Medina, Vincent Castronovo, Xavier Sanjuán, Antonio Cardesa, Fu-Tong Liu, Mark E. Sobel

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Abstract

Galectin-3 is a 31 kD β-galactoside-binding lectin which is expressed by several types of non-neoplastic and neoplastic cells and which may be involved in cell-extracellular matrix interactions. An immunohistochemical study has been made of the expression of galectin-3, as well as its ligand, laminin, in a spectrum of benign and malignant thyroid neoplasms and in some non-neoplastic conditions. Immunonistochemistry with anti-human recombinant galectin-3 antibody showed consistent, intense positivity in the neoplastic cells of 18 cases of papillary carcinoma and less intense staining in the five anaplastic carcinomas studied. In addition, two out of three poorly differentiated carcinomas, three out of six medullary carcinomas, and four out of eight follicular carcinomas had less intense or focal positivity. One case of Hurthle cell carcinoma showed scattered strongly positive cells. Eight follicular adenomas, three hyperplastic nodules, five nodular goitres, and normal thyroid tissue were negative. Galectin-3 mRNA expression was also evaluated in three of the papillary carcinomas, two follicular adenomas, and one hyperplastic nodule with matched normal tissue. Northern blot analysis demonstrated mRNA overexpression in the three cases of papillary carcinomas, whereas normal and benign tissues were negative. Laminin distribution in neoplastic and non-neoplastic tissue varied with architectural patterns but did not correlate with galectin-3 immunonistochemical expression. We conclude that expression of galectin-3 is limited to inflammatory foci in normal and benign thyroid tissue and is a phenotypic feature of malignant thyroid neoplasms, especially papillary carcinomas.

Original languageEnglish (US)
Pages (from-to)80-86
Number of pages7
JournalJournal of Pathology
Volume181
Issue number1
DOIs
StatePublished - Jan 1997

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Galectin 3
Laminin
Papillary Carcinoma
Thyroid Gland
Carcinoma
Thyroid Neoplasms
Adenoma
Oxyphil Cells
Nodular Goiter
Galactosides
Messenger RNA
Medullary Carcinoma
Lectins
Northern Blotting
Extracellular Matrix
Staining and Labeling
Ligands
Antibodies

Keywords

  • carcinoma
  • galectin-3
  • laminin
  • thyroid

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Fernández, P. L., Merino, M. J., Gómez, M., Campo, E., Medina, T., Castronovo, V., ... Sobel, M. E. (1997). Galectin-3 and laminin expression in neoplastic and non-neoplastic thyroid tissue. Journal of Pathology, 181(1), 80-86. https://doi.org/10.1002/(SICI)1096-9896(199701)181:1<80::AID-PATH699>3.0.CO;2-E

Galectin-3 and laminin expression in neoplastic and non-neoplastic thyroid tissue. / Fernández, Pedro L.; Merino, María J.; Gómez, Mercedes; Campo, Elías; Medina, Teresa; Castronovo, Vincent; Sanjuán, Xavier; Cardesa, Antonio; Liu, Fu-Tong; Sobel, Mark E.

In: Journal of Pathology, Vol. 181, No. 1, 01.1997, p. 80-86.

Research output: Contribution to journalArticle

Fernández, PL, Merino, MJ, Gómez, M, Campo, E, Medina, T, Castronovo, V, Sanjuán, X, Cardesa, A, Liu, F-T & Sobel, ME 1997, 'Galectin-3 and laminin expression in neoplastic and non-neoplastic thyroid tissue', Journal of Pathology, vol. 181, no. 1, pp. 80-86. https://doi.org/10.1002/(SICI)1096-9896(199701)181:1<80::AID-PATH699>3.0.CO;2-E
Fernández, Pedro L. ; Merino, María J. ; Gómez, Mercedes ; Campo, Elías ; Medina, Teresa ; Castronovo, Vincent ; Sanjuán, Xavier ; Cardesa, Antonio ; Liu, Fu-Tong ; Sobel, Mark E. / Galectin-3 and laminin expression in neoplastic and non-neoplastic thyroid tissue. In: Journal of Pathology. 1997 ; Vol. 181, No. 1. pp. 80-86.
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AU - Castronovo, Vincent

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