Galectin-3 and galectin-1 bind distinct cell surface glycoprotein receptors to induce T cell death

Brianna N. Stillman, Daniel K. Hsu, Mabel Pang, C. Fred Brewer, Pauline Johnson, Fu-Tong Liu, Linda G. Baum

Research output: Contribution to journalArticle

309 Citations (Scopus)

Abstract

Galectins are a family of mammalian β-galactoside-binding proteins that positively and negatively regulate T cell death. Extracellular galectin-1 directly induces death of T cells and thymocytes, while intracellular galectin-3 blocks T cell death. In contrast to the antiapoptotic function of intracellular galectin-3, we demonstrate that extracellular galectin-3 directly induces death of human thymocytes and T cells. However, events in galectin-3- and galectin-1-induced cell death differ in a number of ways. Thymocyte subsets demonstrate different susceptibility to the two galectins: whereas galectin-1 kills double-negative and double-positive human thymocytes with equal efficiency, galectin-3 preferentially kills double-negative thymocytes. Galectin-3 binds to a complement of T cell surface glycoprotein receptors distinct from that recognized by galectin-1. Of these glycoprotein receptors, CD45 and CD71, but not CD29 and CD43, appear to be involved in galectin-3-induced T cell death. In addition, CD7 that is required for galectin-1-induced death is not required for death triggered by galectin-3. Following galectin-3 binding, CD45 remains uniformly distributed on the cell surface, in contrast to the CD45 clustering induced by galectin-1. Thus, extracellular galectin-3 and galectin-1 induce death of T cells through distinct cell surface events. However, as galectin-3 and galectin-1 cell death are neither additive nor synergistic, the two death pathways may converge inside the cell.

Original languageEnglish (US)
Pages (from-to)778-789
Number of pages12
JournalJournal of Immunology
Volume176
Issue number2
StatePublished - Jan 15 2006

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Galectin 1
Galectin 3
Membrane Glycoproteins
Cell Surface Receptors
Cell Death
T-Lymphocytes
Thymocytes
Galectins
Galactosides
Cluster Analysis
Glycoproteins
Carrier Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Stillman, B. N., Hsu, D. K., Pang, M., Brewer, C. F., Johnson, P., Liu, F-T., & Baum, L. G. (2006). Galectin-3 and galectin-1 bind distinct cell surface glycoprotein receptors to induce T cell death. Journal of Immunology, 176(2), 778-789.

Galectin-3 and galectin-1 bind distinct cell surface glycoprotein receptors to induce T cell death. / Stillman, Brianna N.; Hsu, Daniel K.; Pang, Mabel; Brewer, C. Fred; Johnson, Pauline; Liu, Fu-Tong; Baum, Linda G.

In: Journal of Immunology, Vol. 176, No. 2, 15.01.2006, p. 778-789.

Research output: Contribution to journalArticle

Stillman, BN, Hsu, DK, Pang, M, Brewer, CF, Johnson, P, Liu, F-T & Baum, LG 2006, 'Galectin-3 and galectin-1 bind distinct cell surface glycoprotein receptors to induce T cell death', Journal of Immunology, vol. 176, no. 2, pp. 778-789.
Stillman BN, Hsu DK, Pang M, Brewer CF, Johnson P, Liu F-T et al. Galectin-3 and galectin-1 bind distinct cell surface glycoprotein receptors to induce T cell death. Journal of Immunology. 2006 Jan 15;176(2):778-789.
Stillman, Brianna N. ; Hsu, Daniel K. ; Pang, Mabel ; Brewer, C. Fred ; Johnson, Pauline ; Liu, Fu-Tong ; Baum, Linda G. / Galectin-3 and galectin-1 bind distinct cell surface glycoprotein receptors to induce T cell death. In: Journal of Immunology. 2006 ; Vol. 176, No. 2. pp. 778-789.
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