Galectin-12 is required for adipogenic signaling and adipocyte differentation

Ri Yao Yang, Daniel K. Hsu, Lan Yu, Huan Yuan Chen, Fu-Tong Liu

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Galectin-12 is a member of the galectin family consisting of β-galactoside-binding proteins with conserved carbohydrate recognition domains. This protein is preferentially expressed in peripheral blood leukocytes and adipocytes. We previously showed that galectin-12 is induced by cell cycle block at the G1 phase and causes G1 arrest when overexpressed (Yang, R.-Y., Hsu, D. K., Yu, L., Ni, J., and Liu, F.-T. (2001) J. Biol. Chem. 276, 20252-20260). Here, we show that the galectin-12 gene is expressed in mouse preadipocytes and is up-regulated when preadipocytes undergo cell cycle arrest, concomitant with acquisition of the competence to undergo differentiation in response to adipogenic hormone stimulation. Following a brief down-regulation 1 day after adipogenic treatment, its expression was once again markedly elevated when cells underwent terminal differentiation. Down-regulation of endogenous galectin-12 expression by RNA interference greatly reduced the expression of the adipogenic transcription factors CCAAT/enhancer-binding protein-β and -α and peroxisome proliferator-activated receptor-γ and severely suppressed adipocyte differentiation as a result of defective adipogenic signaling. We conclude that galectin-12 is required for signal transduction that conveys hormone stimulation to the induction of adipogenic factors essential for adipocyte differentiation. The findings suggest that galectin-12 is a major regulator of adipose tissue development.

Original languageEnglish (US)
Pages (from-to)29761-29766
Number of pages6
JournalJournal of Biological Chemistry
Volume279
Issue number28
DOIs
StatePublished - Jul 9 2004

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Galectins
Adipocytes
Down-Regulation
Cells
Hormones
CCAAT-Enhancer-Binding Proteins
Galactosides
Signal transduction
Peroxisome Proliferator-Activated Receptors
G1 Phase
RNA Interference
Cell Cycle Checkpoints
Mental Competency
Adipose Tissue
Signal Transduction
Cell Cycle
Carrier Proteins
Leukocytes
Blood
Transcription Factors

ASJC Scopus subject areas

  • Biochemistry

Cite this

Galectin-12 is required for adipogenic signaling and adipocyte differentation. / Yang, Ri Yao; Hsu, Daniel K.; Yu, Lan; Chen, Huan Yuan; Liu, Fu-Tong.

In: Journal of Biological Chemistry, Vol. 279, No. 28, 09.07.2004, p. 29761-29766.

Research output: Contribution to journalArticle

Yang, Ri Yao ; Hsu, Daniel K. ; Yu, Lan ; Chen, Huan Yuan ; Liu, Fu-Tong. / Galectin-12 is required for adipogenic signaling and adipocyte differentation. In: Journal of Biological Chemistry. 2004 ; Vol. 279, No. 28. pp. 29761-29766.
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