Galectin-12 is Involved in Corn Silk-Induced Anti-Adipogenesis and Anti-Obesity Effects

Yu An Hsu, Yi Hsin Kuo, Chih Sheng Chen, Ying Chi Chen, Chi Chun Huang, Ching Yao Chang, Chao Jen Lin, Cheng Wen Lin, Hui Ju Lin, Fu-Tong Liu, Lei Wan

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Obesity is a significant risk factor for various diseases. It is a clinical condition caused by the excessive accumulation of fat, which has a negative impact on human health. Galactin-12 is an adipocyte-expressed protein and possesses adipocyte-inducing activity. We investigated the expression level of candidate proteins involved in galactin-12-mediated adipocyte differentiation pathway. We performed a high-throughput screening assay to monitor galectin-12 promoter activity using 105 traditional Chinese herbs. Corn silk extract and (Formula presented.)-sitosterol reduced the expression of galactin-12 promoter in 3T3-L1 cells. In addition, corn silk extract and (Formula presented.)-sitosterol decreased the level of lipid droplets and downregulated the gene and protein expression level of C/EBP(Formula presented.), C/EBP(Formula presented.), PPAR(Formula presented.), Ap2, and adipsin in 3T3-L1 pre-adipocytes via AKT and ERK1/2 inhibition. In vivo study with the oral administration of corn silk extract and (Formula presented.)-sitosterol in a mouse model showed a significant weight reduction and decrease in adipocytes in several organs such as the liver and adipose tissue. Taken together, corn silk extract and (Formula presented.)-sitosterol may effectively reduce pre-adipocyte differentiation by inhibiting galectin-12 activity and exerting anti-obesity effects. These findings highlight the potential use of corn silk extract and (Formula presented.)-sitosterol as potential candidates for the prevention and treatment of obesity.

Original languageEnglish (US)
Pages (from-to)1-19
Number of pages19
JournalAmerican Journal of Chinese Medicine
DOIs
StateAccepted/In press - Jul 5 2018

Keywords

  • (Formula presented.)-Sitosterol
  • Adipogenesis
  • Corn Silk
  • Galectin-12
  • Obesity

ASJC Scopus subject areas

  • Complementary and alternative medicine

Fingerprint Dive into the research topics of 'Galectin-12 is Involved in Corn Silk-Induced Anti-Adipogenesis and Anti-Obesity Effects'. Together they form a unique fingerprint.

  • Cite this

    Hsu, Y. A., Kuo, Y. H., Chen, C. S., Chen, Y. C., Huang, C. C., Chang, C. Y., Lin, C. J., Lin, C. W., Lin, H. J., Liu, F-T., & Wan, L. (Accepted/In press). Galectin-12 is Involved in Corn Silk-Induced Anti-Adipogenesis and Anti-Obesity Effects. American Journal of Chinese Medicine, 1-19. https://doi.org/10.1142/S0192415X18500544