Galectin-1 induces nuclear translocation of endonuclease G in caspase- and cytochrome c-independent T cell death

H. P. Hahn, M. Pang, J. He, J. D. Hernandez, R. Y. Yang, L. Y. Li, X. Wang, Fu-Tong Liu, L. G. Baum

Research output: Contribution to journalArticle

112 Citations (Scopus)

Abstract

Galectin-1, a mammalian lectin expressed in many tissues, induces death of diverse cell types, including lymphocytes and tumor cells. The galectin-1 T cell death pathway is novel and distinct from other death pathways, including those initiated by Fas and corticosterolds. We have found that galectin-1 binding to human T cell lines triggered rapid translocation of endonuclease G from mitochondria to nuclei. However, endonuclease G nuclear translocation occurred without cytochrome c release from mitochondria, without nuclear translocation of apoptosis-inducing factor, and prior to loss of mitochondrial membrane potential. Galectin-1 treatment did not result in caspase activation, nor was death blocked by caspase inhibitors. However, galectin-1 cell death was inhibited by intracellular expression of galectin-3, and galectin-3 expression inhibited the eventual loss of mitochondrial membrane potential. Galectin-1-induced cell death proceeds via a caspase-independent pathway that involves a unique pattern of mitochondrial events, and different galectin family members can coordinately regulate susceptibility to cell death.

Original languageEnglish (US)
Pages (from-to)1277-1286
Number of pages10
JournalCell Death and Differentiation
Volume11
Issue number12
DOIs
StatePublished - Dec 2004
Externally publishedYes

Fingerprint

Galectin 1
Caspases
Cell Death
T-Lymphocytes
Galectin 3
Mitochondrial Membrane Potential
Mitochondria
Apoptosis Inducing Factor
Galectins
Caspase Inhibitors
Cytochromes c
Lectins
endonuclease G
cytochrome c-T
Lymphocytes
Cell Line

Keywords

  • Apoptosis
  • Endonuclease G
  • Galectin
  • Human
  • T lymphocyte

ASJC Scopus subject areas

  • Cell Biology

Cite this

Hahn, H. P., Pang, M., He, J., Hernandez, J. D., Yang, R. Y., Li, L. Y., ... Baum, L. G. (2004). Galectin-1 induces nuclear translocation of endonuclease G in caspase- and cytochrome c-independent T cell death. Cell Death and Differentiation, 11(12), 1277-1286. https://doi.org/10.1038/sj.cdd.4401485

Galectin-1 induces nuclear translocation of endonuclease G in caspase- and cytochrome c-independent T cell death. / Hahn, H. P.; Pang, M.; He, J.; Hernandez, J. D.; Yang, R. Y.; Li, L. Y.; Wang, X.; Liu, Fu-Tong; Baum, L. G.

In: Cell Death and Differentiation, Vol. 11, No. 12, 12.2004, p. 1277-1286.

Research output: Contribution to journalArticle

Hahn, HP, Pang, M, He, J, Hernandez, JD, Yang, RY, Li, LY, Wang, X, Liu, F-T & Baum, LG 2004, 'Galectin-1 induces nuclear translocation of endonuclease G in caspase- and cytochrome c-independent T cell death', Cell Death and Differentiation, vol. 11, no. 12, pp. 1277-1286. https://doi.org/10.1038/sj.cdd.4401485
Hahn, H. P. ; Pang, M. ; He, J. ; Hernandez, J. D. ; Yang, R. Y. ; Li, L. Y. ; Wang, X. ; Liu, Fu-Tong ; Baum, L. G. / Galectin-1 induces nuclear translocation of endonuclease G in caspase- and cytochrome c-independent T cell death. In: Cell Death and Differentiation. 2004 ; Vol. 11, No. 12. pp. 1277-1286.
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