GABAA receptor subtype selectivity of the proconvulsant rodenticide TETS

Brandon Pressly, Hai M. Nguyen, Heike Wulff

Research output: Contribution to journalArticlepeer-review

10 Scopus citations


The rodenticide tetramethylenedisulfotetramine (TETS) is a potent convulsant (lethal dose in humans 7–10 mg) that is listed as a possible threat agent by the United States Department of Homeland Security. TETS has previously been studied in vivo for toxicity and in vitro in binding assays, with the latter demonstrating it to be a non-competitive antagonist on GABAA receptors. To determine whether TETS exhibits subtype selectivity for a particular GABAA receptor combination, we used whole-cell patch-clamp to determine the potency of TETS on the major synaptic and extrasynaptic GABAA receptors associated with convulsant activity. The active component of picrotoxin, picrotoxinin, was used as a control. While picrotoxinin did not differentiate well between 13 GABAA receptors, TETS exhibited the highest activity on α2β3γ2 (IC50 480 nM, 95% CI 320–640 nM) and α6β3γ2 (IC50 400 nM, 95% CI 290–510 nM). Introducing β1 or β2 subunits into these receptor combinations reduced or abolished TETS sensitivity, suggesting that TETS preferentially affects receptors with α2/β3 or α6/β3 composition. Since α2β3γ2 receptors make up 15–20% of the GABAA receptors in the mammalian CNS, we suggest that α2β3γ2 is probably the most important GABAA receptor for the seizure-inducing activity of TETS.

Original languageEnglish (US)
Pages (from-to)1-12
Number of pages12
JournalArchives of Toxicology
StateAccepted/In press - Oct 16 2017


  • Convulsant
  • Electrophysiology
  • GABA receptor
  • Picrotoxinin
  • TETS
  • Threat agent

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis


Dive into the research topics of 'GABAA receptor subtype selectivity of the proconvulsant rodenticide TETS'. Together they form a unique fingerprint.

Cite this