G6b-B cell surface inhibitory receptor expression is highly restricted to CD4+ T-cells and induced by interleukin-4-activated STAT6 pathway

Jianfeng Li, Martin Cadeiras, Manuel Prinz von Bayern, Lining Zhang, Adriana I. Colovai, Russell Dedrick, Eric A. Jaffe, Nicole Suciu-Foca, Mario C. Deng

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

The G6b-B gene encodes a novel cell surface receptor of the immunoglobulin superfamily that activates inhibitory signaling pathways by triggering SHP-1/SHP-2 via immunoreceptor tyrosine-based inhibitory motifs (ITIM) in its cytoplasmic domain. We previously identified decreased G6b-B expression in peripheral blood mononuclear cells (PBMC) during acute cellular cardiac allograft rejection. We studied the expression of G6b-B in different human mononuclear cell populations and its regulation. Real-time polymerase chain reaction (PCR) revealed that G6b-B mRNA is higher in CD4+ T cells or monocytes, but is not different between CD25+ CD4+ T cells and CD25- CD4+ T cells. G6b-B mRNA was increased in CD4+ T cells in presence of interleukin-4 in dose- and time-dependent manners. To understand the regulatory mechanism, we analyzed a 1.9-kb 5′-flanking region of the G6b-B translation start site and found a putative cis-acting element for Signal Transducer and Activator of Transcription (STAT)-6. Luciferase-reporter-gene-assay and electrophoretic mobility shift assays identified the STAT6 site as necessary for the induction of G6b-B by IL-4. Our study demonstrates that G6b-B expression is highly restricted to peripheral CD4+ T cells and up-regulated by the IL-4-induced STAT6 pathway, strongly suggesting that G6b-B is involved in regulation of the immune response by CD4+ T cell-mediated and IL-4 induced regulatory mechanisms.

Original languageEnglish (US)
Pages (from-to)708-714
Number of pages7
JournalHuman Immunology
Volume68
Issue number8
DOIs
StatePublished - Aug 1 2007
Externally publishedYes

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Keywords

  • Allograft rejection
  • CD4+ T cells
  • G6b
  • IL-4
  • STAT6
  • Transplantation

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Li, J., Cadeiras, M., Prinz von Bayern, M., Zhang, L., Colovai, A. I., Dedrick, R., Jaffe, E. A., Suciu-Foca, N., & Deng, M. C. (2007). G6b-B cell surface inhibitory receptor expression is highly restricted to CD4+ T-cells and induced by interleukin-4-activated STAT6 pathway. Human Immunology, 68(8), 708-714. https://doi.org/10.1016/j.humimm.2007.04.008