Further optimization of mouse spermatozoa evaporative drying techniques

Heidi Y. Elmoazzen, Gloria Y. Lee, Ming W. Li, Lynda K. McGinnis, Kevin C K Lloyd, Mehmet Toner, John D. Biggers

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

It has been shown in the past that mouse spermatozoa could be dried under a stream of nitrogen gas at ambient temperature and stored at 4 °C or 22 °C for up to 3 months and was capable of generating live-born offspring. In previous desiccation work, dried sperm were stored in a vacuum-sealed plastic bag placed in a vacuum-packed Mylar bag. However, dried specimens stored in this way often lost moisture, particularly in samples stored at higher temperatures (22 °C) compared to lower temperatures (4 °C). The present report describes a method which minimizes this water loss from the dried sperm samples. Its use is described in a preliminary study on the effect of supplementing the trehalose with glycerol. The results have demonstrated that mouse sperm can be stored at 4 °C over saturated NaBr without the uptake of water which occurs when they are stored in Mylar packages. In addition, we were able to get some survival of sperm (9-15%) at room temperature storage after 3 months. The addition of glycerol to trehalose had little effect on the survival of dried mouse sperm stored over NaBr for 1 and 3 months.

Original languageEnglish (US)
Pages (from-to)113-115
Number of pages3
JournalCryobiology
Volume59
Issue number1
DOIs
StatePublished - Aug 2009

Keywords

  • Desiccation
  • Evaporative drying
  • Glycerol
  • Intracytoplasmic injection
  • Long-term storage
  • Mammalian cells
  • Mouse sperm
  • Preservation
  • Temperature
  • Trehalose

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

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  • Cite this

    Elmoazzen, H. Y., Lee, G. Y., Li, M. W., McGinnis, L. K., Lloyd, K. C. K., Toner, M., & Biggers, J. D. (2009). Further optimization of mouse spermatozoa evaporative drying techniques. Cryobiology, 59(1), 113-115. https://doi.org/10.1016/j.cryobiol.2009.03.005