Further characterization of a potent immunogen and the chromosomal gene encoding it in the lyme disease agent, Borrelia burgdorferi

G. C. Perng, Rance B Lefebvre, R. C. Johnson

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

Further characterization of a previously reported 83-kDa antigen of Borrelia burgdorferi and the gene encoding it is reported. The DNA sequence of the gene and the amino acid sequence of the protein were determined. On the basis of the amino acid content, the actual size of the antigen was determined to be 79.8 kDa, rather than 83 kDa as previously reported. The expression of the antigen in several representative North American and European B. burgdorferi isolates was demonstrated. The conservation of the gene within the species was demonstrated by DNA hybridization of a labeled gene probe to several North American and European B. burgdorferi isolates. Of other Borrelia spp. assayed (B. hermsii, B. coriaceae, B. duttonii, and B. anserina), only B. anserina, a poultry pathogen, hybridized to the gene probe and expressed the 79.8-kDa antigen.

Original languageEnglish (US)
Pages (from-to)2070-2074
Number of pages5
JournalInfection and Immunity
Volume59
Issue number6
StatePublished - 1991

ASJC Scopus subject areas

  • Immunology

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