Functions of the Snf2/Swi2 family Rad54 motor protein in homologous recombination

Shannon J. Ceballos, Wolf Dietrich Heyer

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

Homologous recombination is a central pathway to maintain genomic stability and is involved in the repair of DNA damage and replication fork support, as well as accurate chromosome segregation during meiosis. Rad54 is a dsDNA-dependent ATPase of the Snf2/Swi2 family of SF2 helicases, although Rad54 lacks classical helicase activity and cannot carry out the strand displacement reactions typical for DNA helicases. Rad54 is a potent and processive motor protein that translocates on dsDNA, potentially executing several functions in recombinational DNA repair. Rad54 acts in concert with Rad51, the central protein of recombination that performs the key reactions of homology search and DNA strand invasion. Here, we will review the role of the Rad54 protein in homologous recombination with an emphasis on mechanistic studies with the yeast and human enzymes. We will discuss how these results relate to in vivo functions of Rad54 during homologous recombination in somatic cells and during meiosis. This article is part of a Special Issue entitled: Snf2/Swi2 ATPase structure and function.

Original languageEnglish (US)
Pages (from-to)509-523
Number of pages15
JournalBiochimica et Biophysica Acta - Gene Regulatory Mechanisms
Volume1809
Issue number9
DOIs
StatePublished - Sep 2011

Fingerprint

Homologous Recombination
Meiosis
Adenosine Triphosphatases
DNA
Repair
DNA Helicases
Recombinational DNA Repair
Chromosome Segregation
Proteins
Genomic Instability
Chromosomes
DNA Replication
Yeast
Genetic Recombination
DNA Damage
Yeasts
Enzymes

Keywords

  • DNA repair
  • DNA replication
  • Genome stability
  • Homologous recombination
  • Meiosis
  • Motor protein

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Genetics
  • Molecular Biology
  • Structural Biology

Cite this

Functions of the Snf2/Swi2 family Rad54 motor protein in homologous recombination. / Ceballos, Shannon J.; Heyer, Wolf Dietrich.

In: Biochimica et Biophysica Acta - Gene Regulatory Mechanisms, Vol. 1809, No. 9, 09.2011, p. 509-523.

Research output: Contribution to journalArticle

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