Abstract
Two genetically engineered strains of mice were used to characterize murine cone function electroretinographically, without interference of rod-driven responses: (1) mice with a deletion of the gene for the rod transducin α-subunit (transducin α-/-), and (2) mice with rod arrestin deleted (arrestin -/-). In the first three months of age, both strains have a normal complement of rods and normal rod structure, but transducin α-/- mice have no rod-driven responses to light, while rod-driven activity of arrestin -/- mice can be suppressed by a single intense flash for hours. In response to intense flashes the electroretinograms of these strains of mice showed a readily identifiable, pure-cone a-wave of ∼10 μV saturating amplitude. A 530 nm background that saturates rod responses of wild type mice was found to desensitize the b-wave responses of mice of both transgenic lines, whether the b-waves were driven by photons captured by M- or UV-cone pigments. The desensitizing effect of the 530 nm background on UV-pigment driven responses provides new evidence in support of the hypothesis of functional co-expression of the M-pigment in cones expressing primarily the UV-pigment.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 401-415 |
| Number of pages | 15 |
| Journal | Vision Research |
| Volume | 42 |
| Issue number | 4 |
| DOIs | |
| State | Published - 2002 |
| Externally published | Yes |
Fingerprint
Keywords
- Arrestin
- Light adaptation
- Phototransduction
- Transducin
- Transgenic mice
ASJC Scopus subject areas
- Ophthalmology
- Sensory Systems
Cite this
Functionally rodless mice : Transgenic models for the investigation of cone function in retinal disease and therapy. / Lyubarsky, A. L.; Lem, J.; Chen, J.; Falsini, B.; Iannaccone, A.; Pugh Jr, Edward N.
In: Vision Research, Vol. 42, No. 4, 2002, p. 401-415.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Functionally rodless mice
T2 - Transgenic models for the investigation of cone function in retinal disease and therapy
AU - Lyubarsky, A. L.
AU - Lem, J.
AU - Chen, J.
AU - Falsini, B.
AU - Iannaccone, A.
AU - Pugh Jr, Edward N
PY - 2002
Y1 - 2002
N2 - Two genetically engineered strains of mice were used to characterize murine cone function electroretinographically, without interference of rod-driven responses: (1) mice with a deletion of the gene for the rod transducin α-subunit (transducin α-/-), and (2) mice with rod arrestin deleted (arrestin -/-). In the first three months of age, both strains have a normal complement of rods and normal rod structure, but transducin α-/- mice have no rod-driven responses to light, while rod-driven activity of arrestin -/- mice can be suppressed by a single intense flash for hours. In response to intense flashes the electroretinograms of these strains of mice showed a readily identifiable, pure-cone a-wave of ∼10 μV saturating amplitude. A 530 nm background that saturates rod responses of wild type mice was found to desensitize the b-wave responses of mice of both transgenic lines, whether the b-waves were driven by photons captured by M- or UV-cone pigments. The desensitizing effect of the 530 nm background on UV-pigment driven responses provides new evidence in support of the hypothesis of functional co-expression of the M-pigment in cones expressing primarily the UV-pigment.
AB - Two genetically engineered strains of mice were used to characterize murine cone function electroretinographically, without interference of rod-driven responses: (1) mice with a deletion of the gene for the rod transducin α-subunit (transducin α-/-), and (2) mice with rod arrestin deleted (arrestin -/-). In the first three months of age, both strains have a normal complement of rods and normal rod structure, but transducin α-/- mice have no rod-driven responses to light, while rod-driven activity of arrestin -/- mice can be suppressed by a single intense flash for hours. In response to intense flashes the electroretinograms of these strains of mice showed a readily identifiable, pure-cone a-wave of ∼10 μV saturating amplitude. A 530 nm background that saturates rod responses of wild type mice was found to desensitize the b-wave responses of mice of both transgenic lines, whether the b-waves were driven by photons captured by M- or UV-cone pigments. The desensitizing effect of the 530 nm background on UV-pigment driven responses provides new evidence in support of the hypothesis of functional co-expression of the M-pigment in cones expressing primarily the UV-pigment.
KW - Arrestin
KW - Light adaptation
KW - Phototransduction
KW - Transducin
KW - Transgenic mice
UR - http://www.scopus.com/inward/record.url?scp=0036183762&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036183762&partnerID=8YFLogxK
U2 - 10.1016/S0042-6989(01)00214-0
DO - 10.1016/S0042-6989(01)00214-0
M3 - Article
C2 - 11853756
AN - SCOPUS:0036183762
VL - 42
SP - 401
EP - 415
JO - Vision Research
JF - Vision Research
SN - 0042-6989
IS - 4
ER -