Abstract
Trauma to the central and peripheral nervous systems often lead to serious morbidity. Current surgical methods for repairing or replacing such damage have limitations. Tissue engineering offers a potential alternative. Here we show that functionalized α-helical-peptide hydrogels can be used to induce attachment, migration, proliferation and differentiation of murine embryonic neural stem cells (NSCs). Specifically, compared with undecorated gels, those functionalized with Arg-Gly-Asp-Ser (RGDS) peptides increase the proliferative activity of NSCs; promote their directional migration; induce differentiation, with increased expression of microtubule-associated protein-2, and a low expression of glial fibrillary acidic protein; and lead to the formation of larger neurospheres. Electrophysiological measurements from NSCs grown in RGDS-decorated gels indicate developmental progress toward mature neuron-like behavior. Our data indicate that these functional peptide hydrogels may go some way toward overcoming the limitations of current approaches to nerve-tissue repair.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 431-439 |
| Number of pages | 9 |
| Journal | ACS Biomaterial Science and Engineering |
| Volume | 1 |
| Issue number | 6 |
| DOIs | |
| State | Published - Dec 14 2015 |
| Externally published | Yes |
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Keywords
- hydrogel
- nerve tissue engineering
- peptide
- RGD peptide
- self-assembly
- stem cell
ASJC Scopus subject areas
- Biomaterials
- Biomedical Engineering
Cite this
Functionalized α-Helical Peptide Hydrogels for Neural Tissue Engineering. / Mehrban, Nazia; Zhu, Bangfu; Tamagnini, Francesco; Young, Fraser I.; Wasmuth, Alexandra; Hudson, Kieran L.; Thomson, Andrew R.; Birchall, Martin A.; Randall, Andrew D.; Song, Bing; Woolfson, Derek N.
In: ACS Biomaterial Science and Engineering, Vol. 1, No. 6, 14.12.2015, p. 431-439.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Functionalized α-Helical Peptide Hydrogels for Neural Tissue Engineering
AU - Mehrban, Nazia
AU - Zhu, Bangfu
AU - Tamagnini, Francesco
AU - Young, Fraser I.
AU - Wasmuth, Alexandra
AU - Hudson, Kieran L.
AU - Thomson, Andrew R.
AU - Birchall, Martin A.
AU - Randall, Andrew D.
AU - Song, Bing
AU - Woolfson, Derek N.
PY - 2015/12/14
Y1 - 2015/12/14
N2 - Trauma to the central and peripheral nervous systems often lead to serious morbidity. Current surgical methods for repairing or replacing such damage have limitations. Tissue engineering offers a potential alternative. Here we show that functionalized α-helical-peptide hydrogels can be used to induce attachment, migration, proliferation and differentiation of murine embryonic neural stem cells (NSCs). Specifically, compared with undecorated gels, those functionalized with Arg-Gly-Asp-Ser (RGDS) peptides increase the proliferative activity of NSCs; promote their directional migration; induce differentiation, with increased expression of microtubule-associated protein-2, and a low expression of glial fibrillary acidic protein; and lead to the formation of larger neurospheres. Electrophysiological measurements from NSCs grown in RGDS-decorated gels indicate developmental progress toward mature neuron-like behavior. Our data indicate that these functional peptide hydrogels may go some way toward overcoming the limitations of current approaches to nerve-tissue repair.
AB - Trauma to the central and peripheral nervous systems often lead to serious morbidity. Current surgical methods for repairing or replacing such damage have limitations. Tissue engineering offers a potential alternative. Here we show that functionalized α-helical-peptide hydrogels can be used to induce attachment, migration, proliferation and differentiation of murine embryonic neural stem cells (NSCs). Specifically, compared with undecorated gels, those functionalized with Arg-Gly-Asp-Ser (RGDS) peptides increase the proliferative activity of NSCs; promote their directional migration; induce differentiation, with increased expression of microtubule-associated protein-2, and a low expression of glial fibrillary acidic protein; and lead to the formation of larger neurospheres. Electrophysiological measurements from NSCs grown in RGDS-decorated gels indicate developmental progress toward mature neuron-like behavior. Our data indicate that these functional peptide hydrogels may go some way toward overcoming the limitations of current approaches to nerve-tissue repair.
KW - hydrogel
KW - nerve tissue engineering
KW - peptide
KW - RGD peptide
KW - self-assembly
KW - stem cell
UR - http://www.scopus.com/inward/record.url?scp=84969204009&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84969204009&partnerID=8YFLogxK
U2 - 10.1021/acsbiomaterials.5b00051
DO - 10.1021/acsbiomaterials.5b00051
M3 - Article
AN - SCOPUS:84969204009
VL - 1
SP - 431
EP - 439
JO - ACS Biomaterials Science and Engineering
JF - ACS Biomaterials Science and Engineering
SN - 2373-9878
IS - 6
ER -