Functionalized α-Helical Peptide Hydrogels for Neural Tissue Engineering

Nazia Mehrban, Bangfu Zhu, Francesco Tamagnini, Fraser I. Young, Alexandra Wasmuth, Kieran L. Hudson, Andrew R. Thomson, Martin A. Birchall, Andrew D. Randall, Bing Song, Derek N. Woolfson

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Trauma to the central and peripheral nervous systems often lead to serious morbidity. Current surgical methods for repairing or replacing such damage have limitations. Tissue engineering offers a potential alternative. Here we show that functionalized α-helical-peptide hydrogels can be used to induce attachment, migration, proliferation and differentiation of murine embryonic neural stem cells (NSCs). Specifically, compared with undecorated gels, those functionalized with Arg-Gly-Asp-Ser (RGDS) peptides increase the proliferative activity of NSCs; promote their directional migration; induce differentiation, with increased expression of microtubule-associated protein-2, and a low expression of glial fibrillary acidic protein; and lead to the formation of larger neurospheres. Electrophysiological measurements from NSCs grown in RGDS-decorated gels indicate developmental progress toward mature neuron-like behavior. Our data indicate that these functional peptide hydrogels may go some way toward overcoming the limitations of current approaches to nerve-tissue repair.

Original languageEnglish (US)
Pages (from-to)431-439
Number of pages9
JournalACS Biomaterial Science and Engineering
Volume1
Issue number6
DOIs
StatePublished - Dec 14 2015
Externally publishedYes

Fingerprint

Hydrogels
Stem cells
Tissue engineering
Peptides
arginyl-glycyl-aspartyl-serine
Gels
Proteins
Microtubule-Associated Proteins
Glial Fibrillary Acidic Protein
Bioelectric potentials
Neurology
Neurons
Repair
Tissue

Keywords

  • hydrogel
  • nerve tissue engineering
  • peptide
  • RGD peptide
  • self-assembly
  • stem cell

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering

Cite this

Mehrban, N., Zhu, B., Tamagnini, F., Young, F. I., Wasmuth, A., Hudson, K. L., ... Woolfson, D. N. (2015). Functionalized α-Helical Peptide Hydrogels for Neural Tissue Engineering. ACS Biomaterial Science and Engineering, 1(6), 431-439. https://doi.org/10.1021/acsbiomaterials.5b00051

Functionalized α-Helical Peptide Hydrogels for Neural Tissue Engineering. / Mehrban, Nazia; Zhu, Bangfu; Tamagnini, Francesco; Young, Fraser I.; Wasmuth, Alexandra; Hudson, Kieran L.; Thomson, Andrew R.; Birchall, Martin A.; Randall, Andrew D.; Song, Bing; Woolfson, Derek N.

In: ACS Biomaterial Science and Engineering, Vol. 1, No. 6, 14.12.2015, p. 431-439.

Research output: Contribution to journalArticle

Mehrban, N, Zhu, B, Tamagnini, F, Young, FI, Wasmuth, A, Hudson, KL, Thomson, AR, Birchall, MA, Randall, AD, Song, B & Woolfson, DN 2015, 'Functionalized α-Helical Peptide Hydrogels for Neural Tissue Engineering', ACS Biomaterial Science and Engineering, vol. 1, no. 6, pp. 431-439. https://doi.org/10.1021/acsbiomaterials.5b00051
Mehrban, Nazia ; Zhu, Bangfu ; Tamagnini, Francesco ; Young, Fraser I. ; Wasmuth, Alexandra ; Hudson, Kieran L. ; Thomson, Andrew R. ; Birchall, Martin A. ; Randall, Andrew D. ; Song, Bing ; Woolfson, Derek N. / Functionalized α-Helical Peptide Hydrogels for Neural Tissue Engineering. In: ACS Biomaterial Science and Engineering. 2015 ; Vol. 1, No. 6. pp. 431-439.
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