Functional significance of K+ channel β-subunit KCNE3 in auditory neurons

Wenying Wang, Hyo Jeong Kim, Jeong Han Lee, Victor Wong, Choong Ryoul Sihn, Ping Lv, Maria Cristina Perez Flores, Atefeh Mousavi-Nik, Karen Jo Doyle, Yanfang Xu, Ebenezer N. Yamoah

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13 Scopus citations

Abstract

The KCNE3 β-subunit interacts with and regulates the voltage-dependent gating, kinetics, and pharmacology of a variety of Kv channels in neurons. Because a single neuron may express multiple KCNE3 partners, it is impossible to predict the overall functional relevance of the single transmembrane domain peptide on the pore-forming K+ channel subunits with which it associates. In the inner ear, the role of KCNE3 is undefined, despite its association with Meniere disease and tinnitus. To gain insights on the functional significance of KCNE3 in auditory neurons, we examined the properties of spiral ganglion neurons (SGNs) in Kcne3 null mutant neurons relative to their age-matched controls. We demonstrate that null deletion of Kcne3 abolishes characteristic wide variations in the resting membrane potentials of SGNs and yields age-dependent alterations in action potential and firing properties of neurons along the contour of the cochlear axis, in comparison with age-matched wild-type neurons. The properties of basal SGNs were markedly altered in Kcne3-/- mice compared with the wild-type controls; these include reduced action potential latency, amplitude, and increased firing frequency. Analyses of the underlying conductance demonstrate that null mutation of Kcne3 results in enhanced outward K+ currents, which is sufficient to explain the ensuing membrane potential changes. Additionally, we have demonstrated that KCNE3 may regulate the activity of Kv4.2 channels in SGNs. Finally, there were developmentally mediated compensatory changes that occurred such that, by 8 weeks after birth, the electrical properties of the null mutant neurons were virtually indistinguishable from the wild-type neurons, suggesting that ion channel remodeling in auditory neurons progresses beyond hearing onset.

Original languageEnglish (US)
Pages (from-to)16802-16813
Number of pages12
JournalJournal of Biological Chemistry
Volume289
Issue number24
DOIs
StatePublished - Jun 13 2014

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

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    Wang, W., Kim, H. J., Lee, J. H., Wong, V., Sihn, C. R., Lv, P., Perez Flores, M. C., Mousavi-Nik, A., Doyle, K. J., Xu, Y., & Yamoah, E. N. (2014). Functional significance of K+ channel β-subunit KCNE3 in auditory neurons. Journal of Biological Chemistry, 289(24), 16802-16813. https://doi.org/10.1074/jbc.M113.545236