Functional nonequality of the cardiac and skeletal ryanodine receptors

Junichi Nakai, Tatsuya Ogura, Feliciano Protasi, Clara Franzini-Armstrong, Paul D. Allen, Kurt G. Beam

Research output: Contribution to journalArticle

83 Citations (Scopus)

Abstract

Dihydropyridine receptors (DHPRs), which are voltage-gated Ca2+ channels, and ryanodine receptors (RyRs), which are intracellular Ca2+ release channels, are expressed in diverse cell types, including skeletal and cardiac muscle. In skeletal muscle, there appears to be reciprocal signaling between the skeletal isoforms of both the DHPR and the RyR (RyR-1), such that Ca2+ release activity of RyR-1 is controlled by the DHPR and Ca2+ channel activity of the DHPR is controlled by RyR-1. Dyspedic skeletal muscle cells, which do not express RyR-1, lack excitation-contraction coupling and have an ≃30-fold reduction in L-type Ca2+ current density. Here we have examined the ability of the predominant cardiac and brain RyR isoform, RyR-2, to substitute for RyR-1 in interacting with the skeletal DHPR. When RyR-2 is expressed in dyspedic muscle cells, it gives rise to spontaneous intracellular Ca2+ oscillations and supports Ca2+ entry-induced Ca2+ release. However, unlike RyR-1, the expressed RyR-2 does not increase the Ca2+ channel activity of the DHPR, nor is the gating of RyR-2 controlled by the skeletal DHPR. Thus, the ability to participate in skeletal-type reciprocal signaling appears to be a unique feature of RyR-1.

Original languageEnglish (US)
Pages (from-to)1019-1022
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume94
Issue number3
DOIs
StatePublished - Feb 4 1997
Externally publishedYes

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L-Type Calcium Channels
Ryanodine Receptor Calcium Release Channel
Skeletal Muscle
Muscle Cells
Protein Isoforms
Excitation Contraction Coupling
Myocardium
Brain

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Functional nonequality of the cardiac and skeletal ryanodine receptors. / Nakai, Junichi; Ogura, Tatsuya; Protasi, Feliciano; Franzini-Armstrong, Clara; Allen, Paul D.; Beam, Kurt G.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, No. 3, 04.02.1997, p. 1019-1022.

Research output: Contribution to journalArticle

Nakai, Junichi ; Ogura, Tatsuya ; Protasi, Feliciano ; Franzini-Armstrong, Clara ; Allen, Paul D. ; Beam, Kurt G. / Functional nonequality of the cardiac and skeletal ryanodine receptors. In: Proceedings of the National Academy of Sciences of the United States of America. 1997 ; Vol. 94, No. 3. pp. 1019-1022.
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