Functional imaging of hippocampal place cells at cellular resolution during virtual navigation

Daniel A. Dombeck, Christopher D. Harvey, Lin Tian, Loren L. Looger, David W. Tank

Research output: Contribution to journalArticle

371 Citations (Scopus)

Abstract

Spatial navigation is often used as a behavioral task in studies of the neuronal circuits that underlie cognition, learning and memory in rodents. The combination of in vivo microscopy with genetically encoded indicators has provided an important new tool for studying neuronal circuits, but has been technically difficult to apply during navigation. Here we describe methods for imaging the activity of neurons in the CA1 region of the hippocampus with subcellular resolution in behaving mice. Neurons that expressed the genetically encoded calcium indicator GCaMP3 were imaged through a chronic hippocampal window. Head-restrained mice performed spatial behaviors in a setup combining a virtual reality system and a custom-built two-photon microscope. We optically identified populations of place cells and determined the correlation between the location of their place fields in the virtual environment and their anatomical location in the local circuit. The combination of virtual reality and high-resolution functional imaging should allow a new generation of studies to investigate neuronal circuit dynamics during behavior.

Original languageEnglish (US)
Pages (from-to)1433-1440
Number of pages8
JournalNature Neuroscience
Volume13
Issue number11
DOIs
StatePublished - Nov 2010
Externally publishedYes

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Spatial Behavior
User-Computer Interface
Neurons
Photons
Cognition
Rodentia
Hippocampus
Head
Learning
Calcium
Population
Place Cells
Intravital Microscopy
Spatial Navigation

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Functional imaging of hippocampal place cells at cellular resolution during virtual navigation. / Dombeck, Daniel A.; Harvey, Christopher D.; Tian, Lin; Looger, Loren L.; Tank, David W.

In: Nature Neuroscience, Vol. 13, No. 11, 11.2010, p. 1433-1440.

Research output: Contribution to journalArticle

Dombeck, Daniel A. ; Harvey, Christopher D. ; Tian, Lin ; Looger, Loren L. ; Tank, David W. / Functional imaging of hippocampal place cells at cellular resolution during virtual navigation. In: Nature Neuroscience. 2010 ; Vol. 13, No. 11. pp. 1433-1440.
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