Functional fibrous polypropylene solid support and its application in solid phase peptide synthesis and cell specific binding

Bei Xiang, Kit Lam, Gang Sun

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Surfaces of polypropylene (PP) fabrics were modified through thermal initiated radical graft polymerization with acrylic acid and poly (ethylene glycol) di-acrylate (PEG-DIA). Various polymerization conditions were studied to determine the optimal parameters leading to the best surface functionality. Carboxylic acid groups on the functionalized PP support were further derivatized for solid phase peptide synthesis. Peptide ligands LHPQF and sppLDI were successfully synthesized through simple coupling reactions utilizing Fmoc chemistry. Specific binding of Streptavidin-Alkaline phosphatase conjugate and Jurkat (T lymphoblastic) cell were successfully achieved on the novel functional PP substrates. The good geometrical flexibility, mechanical stability, chemical reactivity and biocompatibility of this novel planar support make it very promising substrate for various biotechnological applications.

Original languageEnglish (US)
Pages (from-to)905-914
Number of pages10
JournalReactive and Functional Polymers
Volume69
Issue number12
DOIs
StatePublished - Dec 2009

Fingerprint

Solid-Phase Synthesis Techniques
Polypropylenes
polymerization
peptide
Peptides
carbopol 940
substrate
Polymerization
carboxylic acid
phosphatase
ethylene
ligand
Chemical reactivity
Streptavidin
Ethylene Glycol
Mechanical stability
Phosphatases
Substrates
Carboxylic Acids
Carboxylic acids

Keywords

  • Peptide synthesis
  • Polypropylene fibers
  • Radical grafting
  • Solid support

ASJC Scopus subject areas

  • Materials Chemistry
  • Polymers and Plastics
  • Chemistry(all)
  • Chemical Engineering(all)
  • Environmental Chemistry
  • Biochemistry

Cite this

Functional fibrous polypropylene solid support and its application in solid phase peptide synthesis and cell specific binding. / Xiang, Bei; Lam, Kit; Sun, Gang.

In: Reactive and Functional Polymers, Vol. 69, No. 12, 12.2009, p. 905-914.

Research output: Contribution to journalArticle

@article{a5b1a3cb38cc4d55b7f8ebb21822f4be,
title = "Functional fibrous polypropylene solid support and its application in solid phase peptide synthesis and cell specific binding",
abstract = "Surfaces of polypropylene (PP) fabrics were modified through thermal initiated radical graft polymerization with acrylic acid and poly (ethylene glycol) di-acrylate (PEG-DIA). Various polymerization conditions were studied to determine the optimal parameters leading to the best surface functionality. Carboxylic acid groups on the functionalized PP support were further derivatized for solid phase peptide synthesis. Peptide ligands LHPQF and sppLDI were successfully synthesized through simple coupling reactions utilizing Fmoc chemistry. Specific binding of Streptavidin-Alkaline phosphatase conjugate and Jurkat (T lymphoblastic) cell were successfully achieved on the novel functional PP substrates. The good geometrical flexibility, mechanical stability, chemical reactivity and biocompatibility of this novel planar support make it very promising substrate for various biotechnological applications.",
keywords = "Peptide synthesis, Polypropylene fibers, Radical grafting, Solid support",
author = "Bei Xiang and Kit Lam and Gang Sun",
year = "2009",
month = "12",
doi = "10.1016/j.reactfunctpolym.2009.09.007",
language = "English (US)",
volume = "69",
pages = "905--914",
journal = "Reactive and Functional Polymers",
issn = "1381-5148",
publisher = "Elsevier",
number = "12",

}

TY - JOUR

T1 - Functional fibrous polypropylene solid support and its application in solid phase peptide synthesis and cell specific binding

AU - Xiang, Bei

AU - Lam, Kit

AU - Sun, Gang

PY - 2009/12

Y1 - 2009/12

N2 - Surfaces of polypropylene (PP) fabrics were modified through thermal initiated radical graft polymerization with acrylic acid and poly (ethylene glycol) di-acrylate (PEG-DIA). Various polymerization conditions were studied to determine the optimal parameters leading to the best surface functionality. Carboxylic acid groups on the functionalized PP support were further derivatized for solid phase peptide synthesis. Peptide ligands LHPQF and sppLDI were successfully synthesized through simple coupling reactions utilizing Fmoc chemistry. Specific binding of Streptavidin-Alkaline phosphatase conjugate and Jurkat (T lymphoblastic) cell were successfully achieved on the novel functional PP substrates. The good geometrical flexibility, mechanical stability, chemical reactivity and biocompatibility of this novel planar support make it very promising substrate for various biotechnological applications.

AB - Surfaces of polypropylene (PP) fabrics were modified through thermal initiated radical graft polymerization with acrylic acid and poly (ethylene glycol) di-acrylate (PEG-DIA). Various polymerization conditions were studied to determine the optimal parameters leading to the best surface functionality. Carboxylic acid groups on the functionalized PP support were further derivatized for solid phase peptide synthesis. Peptide ligands LHPQF and sppLDI were successfully synthesized through simple coupling reactions utilizing Fmoc chemistry. Specific binding of Streptavidin-Alkaline phosphatase conjugate and Jurkat (T lymphoblastic) cell were successfully achieved on the novel functional PP substrates. The good geometrical flexibility, mechanical stability, chemical reactivity and biocompatibility of this novel planar support make it very promising substrate for various biotechnological applications.

KW - Peptide synthesis

KW - Polypropylene fibers

KW - Radical grafting

KW - Solid support

UR - http://www.scopus.com/inward/record.url?scp=70350574130&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70350574130&partnerID=8YFLogxK

U2 - 10.1016/j.reactfunctpolym.2009.09.007

DO - 10.1016/j.reactfunctpolym.2009.09.007

M3 - Article

VL - 69

SP - 905

EP - 914

JO - Reactive and Functional Polymers

JF - Reactive and Functional Polymers

SN - 1381-5148

IS - 12

ER -