Functional domains of the v-erbA protein necessary for oncogenesis are required for transcriptional activation in Saccharomyces cerevisiae

Zeljka McBride, Martin L. Privalsky

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The v-erbA oncogene is a retrovirus-transduced and altered copy of a cellular gene (c-erbA-α) for a thyroid hormone receptor. In this paper we show that the v-erbA domains required for transcriptional activation in yeast and for oncogenic function in animal cells are closely congruent. We conclude that the behavior of the v-erbA protein as a transcriptional activator in yeast appears to closely reflect the same biochemical requirements that are necessary for v-erbA function in the neoplastic vertebrate cell. Intriguingly, parallel analyses of c-erbA-α and -β demonstrated unexpected differences in the activities of the two thyroid hormone receptor isoforms in the yeast, perhaps reflecting different functions of these genes in vertebrates. Furthermore, results obtained by analysis of chimeric v-/c-erbA genes suggest that the basal and the hormone-induced transcriptional activity of the nuclear hormone receptors can be modulated independently by distinct structural features within the protein molecule.

Original languageEnglish (US)
Pages (from-to)1465-1475
Number of pages11
JournalOncogene
Volume8
Issue number6
StatePublished - Jun 1993

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Oncogene Proteins v-erbA
erbA Genes
Transcriptional Activation
Saccharomyces cerevisiae
Carcinogenesis
Thyroid Hormone Receptors
Yeasts
Vertebrates
Retroviridae
Cytoplasmic and Nuclear Receptors
Protein Isoforms
Hormones
Genes
Proteins

ASJC Scopus subject areas

  • Cancer Research
  • Genetics
  • Molecular Biology

Cite this

Functional domains of the v-erbA protein necessary for oncogenesis are required for transcriptional activation in Saccharomyces cerevisiae. / McBride, Zeljka; Privalsky, Martin L.

In: Oncogene, Vol. 8, No. 6, 06.1993, p. 1465-1475.

Research output: Contribution to journalArticle

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