Functional differentiation of cooperating kinesin-2 motors orchestrates cargo import and transport in C. elegans cilia

Bram Prevo, Pierre Mangeol, Felix Oswald, Jonathan M. Scholey, Erwin J G Peterman

Research output: Contribution to journalArticle

69 Scopus citations

Abstract

Intracellular transport depends on cooperation between distinct motor proteins. Two anterograde intraflagellar transport (IFT) motors, heterotrimeric kinesin-II and homodimeric OSM-3, cooperate to move cargo along Caenorhabditis elegans cilia. Here, using quantitative fluorescence microscopy, with single-molecule sensitivity, of IFT in living strains containing single-copy transgenes encoding fluorescent IFT proteins, we show that kinesin-II transports IFT trains through the ciliary base and transition zone to a "handover zone" on the proximal axoneme. There, OSM-3 gradually replaces kinesin-II, yielding velocity profiles inconsistent with in vitro motility assays, and then drives transport to the ciliary tip. Dissociated kinesin-II motors undergo rapid turnaround and recycling to the ciliary base, whereas OSM-3 is recycled mainly to the handover zone. This reveals a functional differentiation in which the slower, less processive kinesin-II imports IFT trains into the cilium and OSM-3 drives their long-range transport, thereby optimizing cargo delivery.

Original languageEnglish (US)
Pages (from-to)1536-1545
Number of pages10
JournalNature Cell Biology
Volume17
Issue number12
DOIs
StatePublished - Nov 27 2015

ASJC Scopus subject areas

  • Cell Biology

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