Abstract
Stem cell-derived cardiomyocytes represent unique tools for cell- and tissue-based regenerative therapies, drug discovery and safety, and studies of fundamental heart-failure mechanisms. However, the degree to which stem cell-derived cardiomyocytes compare to mature cardiomyocytes is often debated. We reasoned that physiological metrics of engineered cardiac tissues offer a means of comparison. We built laminar myocardium engineered from cardiomyocytes that were differentiated from mouse embryonic stem cell-derived cardiac progenitors or harvested directly from neonatal mouse ventricles, and compared their anatomy and physiology in vitro. Tissues assembled from progenitor-derived myocytes and neonate myocytes demonstrated similar cytoskeletal architectures but different gap junction organization and electromechanical properties. Progenitor-derived myocardium had significantly less contractile stress and slower longitudinal conduction velocity than neonate-derived myocardium, indicating that the developmental state of the cardiomyocytes affects the electromechanical function of the resultant engineered tissue. These data suggest a need to establish performance metrics for future stem cell applications.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 387-396 |
| Number of pages | 10 |
| Journal | Stem Cell Reports |
| Volume | 1 |
| Issue number | 5 |
| DOIs | |
| State | Published - Nov 19 2013 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry
- Cell Biology
- Developmental Biology
- Genetics
Cite this
Functional differences in engineered myocardium from embryonic stem cell-derived versus neonatal cardiomyocytes. / Feinberg, Adam W.; Ripplinger, Crystal M; Van Der Meer, Peter; Sheehy, Sean P.; Domian, Ibrahim; Chien, Kenneth R.; Parker, Kevin Kit.
In: Stem Cell Reports, Vol. 1, No. 5, 19.11.2013, p. 387-396.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Functional differences in engineered myocardium from embryonic stem cell-derived versus neonatal cardiomyocytes
AU - Feinberg, Adam W.
AU - Ripplinger, Crystal M
AU - Van Der Meer, Peter
AU - Sheehy, Sean P.
AU - Domian, Ibrahim
AU - Chien, Kenneth R.
AU - Parker, Kevin Kit
PY - 2013/11/19
Y1 - 2013/11/19
N2 - Stem cell-derived cardiomyocytes represent unique tools for cell- and tissue-based regenerative therapies, drug discovery and safety, and studies of fundamental heart-failure mechanisms. However, the degree to which stem cell-derived cardiomyocytes compare to mature cardiomyocytes is often debated. We reasoned that physiological metrics of engineered cardiac tissues offer a means of comparison. We built laminar myocardium engineered from cardiomyocytes that were differentiated from mouse embryonic stem cell-derived cardiac progenitors or harvested directly from neonatal mouse ventricles, and compared their anatomy and physiology in vitro. Tissues assembled from progenitor-derived myocytes and neonate myocytes demonstrated similar cytoskeletal architectures but different gap junction organization and electromechanical properties. Progenitor-derived myocardium had significantly less contractile stress and slower longitudinal conduction velocity than neonate-derived myocardium, indicating that the developmental state of the cardiomyocytes affects the electromechanical function of the resultant engineered tissue. These data suggest a need to establish performance metrics for future stem cell applications.
AB - Stem cell-derived cardiomyocytes represent unique tools for cell- and tissue-based regenerative therapies, drug discovery and safety, and studies of fundamental heart-failure mechanisms. However, the degree to which stem cell-derived cardiomyocytes compare to mature cardiomyocytes is often debated. We reasoned that physiological metrics of engineered cardiac tissues offer a means of comparison. We built laminar myocardium engineered from cardiomyocytes that were differentiated from mouse embryonic stem cell-derived cardiac progenitors or harvested directly from neonatal mouse ventricles, and compared their anatomy and physiology in vitro. Tissues assembled from progenitor-derived myocytes and neonate myocytes demonstrated similar cytoskeletal architectures but different gap junction organization and electromechanical properties. Progenitor-derived myocardium had significantly less contractile stress and slower longitudinal conduction velocity than neonate-derived myocardium, indicating that the developmental state of the cardiomyocytes affects the electromechanical function of the resultant engineered tissue. These data suggest a need to establish performance metrics for future stem cell applications.
UR - http://www.scopus.com/inward/record.url?scp=84888137996&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84888137996&partnerID=8YFLogxK
U2 - 10.1016/j.stemcr.2013.10.004
DO - 10.1016/j.stemcr.2013.10.004
M3 - Article
C2 - 24286027
AN - SCOPUS:84888137996
VL - 1
SP - 387
EP - 396
JO - Stem Cell Reports
JF - Stem Cell Reports
SN - 2213-6711
IS - 5
ER -