Functional comparison of RGS9 splice isoforms in a living cell

Kirill A. Martemyanov, Claudia M. Krispel, Polina V. Lishko, Marie E Burns, Vadim Y. Arshavsky

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Two isoforms of the GTPase-activating protein, regulator of G protein signaling 9 (RGS9), control such fundamental functions as vision and behavior. RGS9-1 regulates phototransduction in rods and cones, and RGS9-2 regulates dopamine and opioid signaling in the basal ganglia. To determine their functional differences in the same intact cell, we replaced RGS9-1 with RGS9-2 in mouse rods. Surprisingly, RGS9-2 not only supported normal photoresponse recovery under moderate light conditions but also outperformed RGS9-1 in bright light. This versatility of RGS9-2 results from its ability to inactivate the G protein, transducin, regardless of its effector interactions, whereas RGS9-1 prefers the G protein-effector complex. Such versatility makes RGS9-2 an isoform advantageous for timely signal inactivation across a wide range of stimulus strengths and may explain its predominant representation throughout the nervous system.

Original languageEnglish (US)
Pages (from-to)20988-20993
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume105
Issue number52
DOIs
StatePublished - Dec 30 2008

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GTP-Binding Protein Regulators
Protein Isoforms
GTP-Binding Proteins
Transducin
Light Signal Transduction
GTPase-Activating Proteins
Light
Vertebrate Photoreceptor Cells
Aptitude
Basal Ganglia
Opioid Analgesics
Nervous System
Dopamine

Keywords

  • G proteins
  • Phototransduction
  • RGS proteins

ASJC Scopus subject areas

  • General

Cite this

Functional comparison of RGS9 splice isoforms in a living cell. / Martemyanov, Kirill A.; Krispel, Claudia M.; Lishko, Polina V.; Burns, Marie E; Arshavsky, Vadim Y.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, No. 52, 30.12.2008, p. 20988-20993.

Research output: Contribution to journalArticle

Martemyanov, Kirill A. ; Krispel, Claudia M. ; Lishko, Polina V. ; Burns, Marie E ; Arshavsky, Vadim Y. / Functional comparison of RGS9 splice isoforms in a living cell. In: Proceedings of the National Academy of Sciences of the United States of America. 2008 ; Vol. 105, No. 52. pp. 20988-20993.
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